Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran ; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Bioimpacts. 2014;4(1):39-42. doi: 10.5681/bi.2014.005. Epub 2014 Feb 5.
Alendronate sodium enhances bone formation and increases osteoblast proliferation and maturation and leads to the inhibition of osteoblast apoptosis. Therefore, a rapid and simple spectrofluorometric method has been developed and validated for the quantitative determination of it.
The procedure is based on the reaction of primary amino group of alendronate with o-phthalaldehyde (OPA) in sodium hydroxide solution.
The calibration graph was linear over the concentration range of 0.0-2.4 μM and limit of detection and limit of quantification of the method was 8.89 and 29 nanomolar, respectively. The enthalpy and entropy of the reaction between alendronate sodium and OPA showed that the reaction is endothermic and entropy favored (ΔH = 154.08 kJ/mol; ΔS = 567.36 J/mol K) which indicates that OPA interaction with alendronate is increased at elevated temperature.
This simple method can be used as a practical technique for the analysis of alendronate in various samples.
阿仑膦酸钠能促进骨形成，增加成骨细胞增殖和成熟，并抑制成骨细胞凋亡。因此，建立并验证了一种快速、简单的分光荧光法来定量测定阿仑膦酸钠。
该方法基于阿仑膦酸钠的伯氨基与邻苯二甲醛（OPA）在氢氧化钠溶液中的反应。
校准曲线在 0.0-2.4 μM 浓度范围内呈线性，方法的检出限和定量限分别为 8.89 和 29 纳摩尔。阿仑膦酸钠与 OPA 之间反应的焓和熵表明，该反应是吸热的，熵有利（ΔH = 154.08 kJ/mol；ΔS = 567.36 J/mol K），这表明 OPA 与阿仑膦酸钠的相互作用在高温下增加。
该简单方法可作为分析各种样品中阿仑膦酸钠的实用技术。
