Kashanian Soheila, Ezzati Nazhad Dolatabadi Jafar
Faculty of Chemistry, Sensor and Biosensor Research Center, Razi University , Kermanshah, Islamic Republic of Iran .
DNA Cell Biol. 2009 Oct;28(10):535-40. doi: 10.1089/dna.2009.0906.
The interaction of native calf thymus DNA with butylated hydroxyanisole (BHA) at physiological pH was investigated by spectrofluorometric, voltammetric, and viscosimetric techniques. BHA molecules intercalated between base pairs of DNA, demonstrated by a sharp increase in specific viscosity of DNA and decreases in the fluorescence of BHA solutions in the presence of increasing amounts of DNA and calculated formation constant (K(f)) at different temperatures. Further, the enthalpy and entropy of the reaction between BHA and calf thymus DNA showed that the reaction is exothermic and enthalpy favored (DeltaH = -89.02 kJ/mol; DeltaS = -211.21 J/mol K). Also, peak current decrease and positive shift in the cyclic voltammetry indicate that BHA is able to intercalate in the DNA base pairs.
采用荧光光谱法、伏安法和粘度法研究了天然小牛胸腺DNA与丁基羟基茴香醚（BHA）在生理pH值下的相互作用。BHA分子插入DNA碱基对之间，这通过DNA比粘度的急剧增加以及在DNA量增加时BHA溶液荧光的降低和不同温度下计算得到的形成常数（K(f)）得以证明。此外，BHA与小牛胸腺DNA反应的焓和熵表明该反应是放热的且焓有利（ΔH = -89.02 kJ/mol；ΔS = -211.21 J/mol K）。而且，循环伏安法中峰值电流降低和正向偏移表明BHA能够插入DNA碱基对中。
