Proskurikov S Ia, Gabaĭ V L, Mosin A F, Riabchenko N I
Tsitologiia. 1989 Jun;31(6):690-5.
After a 3-hour incubation of the Ehrlich ascite tumor cells in buffered Hanks solution, without glucose and oxygen, the extensive cell injuries were observed. The time-course of appearance of these injuries was as follows: cell blebbing, staining of the cells with trypan blue, and then their staining with ethidium bromide. The DNA degradation registered with fluorometric method coincided in time with cell staining with trypan blue. All injuries (except DNA degradation) were delayed at pH 6.0 compared with those at pH 7.3. Glucose added to the cell suspension greatly protected the cells from these injuries, although DNA degradation at pH 6.0 in these conditions was a little higher than that at pH 7.3.
将艾氏腹水瘤细胞在不含葡萄糖和氧气的缓冲汉克斯溶液中孵育3小时后，观察到广泛的细胞损伤。这些损伤出现的时间进程如下：细胞起泡，细胞被台盼蓝染色，然后被溴化乙锭染色。用荧光法测定的DNA降解在时间上与细胞被台盼蓝染色一致。与pH 7.3时相比，在pH 6.0时所有损伤（除DNA降解外）均延迟出现。向细胞悬液中添加葡萄糖可极大地保护细胞免受这些损伤，尽管在这些条件下pH 6.0时的DNA降解略高于pH 7.3时。
