Toshchakov V Iu, Danilov M A, Shestakova N K, Kozlovskaia T I, Kulikova E Iu
Vopr Med Khim. 1989 Jul-Aug;35(4):134-7.
Isolation of individual hepatocytes suspension from rats was carried out using trypsin, collagenase and collalytine; maximal yield of the viable hepatocytes was obtained when 0.05% solution of collagenase was used. Trypsin was ineffective in production of viable cells, while collalytine from pig pancreas exhibited the satisfactory results at concentration of 0.15-0.20%.
采用胰蛋白酶、胶原酶和胶原肽酶从大鼠中分离单个肝细胞悬液;使用0.05%的胶原酶溶液时,活肝细胞的产量最高。胰蛋白酶在产生活细胞方面无效,而猪胰腺来源的胶原肽酶在浓度为0.15 - 0.20%时表现出令人满意的结果。