Niedecken H W, Lutz G, Uerlich M, Bauer R
Universitäts-Hautklinik und Poliklinik, Rheinische Friedrich-Wilhelms-Universität Bonn.
Z Hautkr. 1989 Sep 15;64(9):798-800, 803-4.
183 biopsies taken from various skin diseases as well as normal skin were studied in cryostat sections by means of the avidin-biotin complex immunoperoxidase technique, using monoclonal antibody anti-Leu 6, which is directed against the Langerhans cell antigen CD1. In 47 cases, we observed a reticular, pemphigus-like intercellular pattern. This pattern was neither correlated to individual skin diseases, nor to the quality or quantity of corial or epidermotropic infiltrates, nor to the HLA-DR expression of keratinocytes, nor to the count of Langerhans cells. There was, however, a correlation to the presence of Langerhans cells: This pattern was never observed in the aerosyringia or deep infundibula. We conclude, therefore, that the stained antigen has to be regarded as free CD1 derived from the Langerhans cell and diffusing into the keratinocytic intercellular spaces.
采用抗Leu 6单克隆抗体，运用抗生物素蛋白-生物素复合物免疫过氧化物酶技术，对取自各种皮肤疾病及正常皮肤的183份活检标本进行冰冻切片研究，该抗体针对朗格汉斯细胞抗原CD1。在47例病例中，我们观察到一种网状、类天疱疮样的细胞间模式。这种模式既与个体皮肤疾病无关，也与真皮或亲表皮浸润的性质或数量无关，与角质形成细胞的HLA-DR表达无关，也与朗格汉斯细胞计数无关。然而，它与朗格汉斯细胞的存在有关：在汗腺导管或深部漏斗部从未观察到这种模式。因此，我们得出结论，染色的抗原必须被视为源自朗格汉斯细胞并扩散到角质形成细胞间间隙的游离CD1。
