Section for Endocrinology, Department of Clinical Science, University of Bergen, N-5020 Bergen, Norway.
Section for Endocrinology, Department of Clinical Science, University of Bergen, N-5020 Bergen, Norway.
Clin Immunol. 2014 Jul;153(1):220-7. doi: 10.1016/j.clim.2014.04.013. Epub 2014 May 2.
An important characteristic of autoimmune polyendocrine syndrome type 1 (APS 1) is the existence of neutralizing autoantibodies (nAbs) against the type I interferons (IFN) -α2 and -ω at frequencies close to 100%. Type 1 IFN autoantibodies are detected by antiviral neutralizing assays (AVA), binding assays with radiolabelled antigens (RLBA), enzyme-linked immunosorbent assay (ELISA), or by reporter-based cell assays. We here present a simple and reliable version of the latter utilizing a commercially available cell line (HEK-Blue IFN-α/β). All 67 APS 1 patients were positive for IFN-ω nAbs, while 90% were positive for IFN-α2 nAbs, a 100% and 96% correlation with RLBA, respectively. All blood donors and non-APS 1 patients were negative. The dilution titer required to reduce the effect of IFN-ω nAbs correlated with the RLBA index. This cell-based autoantibody assay (CBAA) is easy to perform, suitable for high throughput, while providing high specificity and sensitivity.
自身免疫性多内分泌腺综合征 1 型（APS1）的一个重要特征是存在针对 I 型干扰素（IFN）-α2 和 -ω 的中和自身抗体（nAbs），其频率接近 100%。1 型 IFN 自身抗体通过抗病毒中和测定法（AVA）、放射性标记抗原结合测定法（RLBA）、酶联免疫吸附测定法（ELISA）或基于报告基因的细胞测定法进行检测。我们在此介绍了一种简单可靠的后者版本，利用市售细胞系（HEK-Blue IFN-α/β）。所有 67 名 APS1 患者的 IFN-ω nAbs 均为阳性，而 IFN-α2 nAbs 的阳性率为 90%，与 RLBA 的相关性分别为 100%和 96%。所有献血者和非 APS1 患者均为阴性。降低 IFN-ω nAbs 作用所需的稀释滴度与 RLBA 指数相关。这种基于细胞的自身抗体测定法（CBAA）易于操作，适合高通量，同时具有高特异性和灵敏度。
