从牛临床乳腺炎中分离金黄色葡萄球菌nuc突变株。
Isolation of nuc mutant isolates of Staphylococcus aureus from bovine clinical mastitis.
作者信息
Zastempowska E, Orczykowska-Kotyna M, Lassa H
机构信息
Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland.
Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland.
出版信息
Vet J. 2014 Jun;200(3):446-8. doi: 10.1016/j.tvjl.2014.03.029. Epub 2014 Mar 31.
Isolates of Staphylococcus aureus with a mutation in the nuclease (nuc) gene were recovered from cases of bovine mastitis in Poland. Three S. aureus isolates from cows in one herd had a 42 base pair duplication in the nuc gene. These isolates belonged to sequence type 97 (ST97) and clonal complex 97 (CC97). They had a different spa type and multiple-locus variable-number tandem-repeat fingerprinting (MLVF) subtype than a S. aureus isolate without the nuc mutation from the same herd. Isolation of nuc mutant S. aureus strains from cases of bovine mastitis may confound diagnostic PCRs based on detection of the nuc gene.
在波兰，从牛乳腺炎病例中分离出了核酸酶（nuc）基因突变的金黄色葡萄球菌菌株。来自一个牛群中奶牛的三株金黄色葡萄球菌在nuc基因中存在42个碱基对的重复。这些分离株属于序列类型97（ST97）和克隆复合体97（CC97）。与同一牛群中未发生nuc基因突变的金黄色葡萄球菌分离株相比，它们具有不同的spa类型和多位点可变数目串联重复指纹图谱（MLVF）亚型。从牛乳腺炎病例中分离出nuc突变的金黄色葡萄球菌菌株可能会干扰基于nuc基因检测的诊断性PCR。
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