Institute for Microbiology, University of Greifswald, Greifswald, Germany.
Proteomics. 2011 Jun;11(12):2491-502. doi: 10.1002/pmic.201000698. Epub 2011 May 18.
Staphylococcus aureus colonizes and infects humans as well as animals. In the present study, 17 S. aureus strains isolated from cows suffering from mastitis were characterized. The well-established multilocus sequence typing (MLST) technique and a diagnostic microarray covering 185 S. aureus virulence and resistance genes were used for genetic and epidemiological analyses. Virulence gene expression studies were performed by analyzing the extracellular protein pattern of each isolate on 2-D gels. By this way, a pronounced heterogeneity of the extracellular proteome between the bovine isolates has been observed which was attributed to genome plasticity and variation of gene expression. Merely 12 proteins were expressed in at least 80% of the isolates, i.e. Atl, Aur, GlpQ, Hla, LtaS, Nuc, PdhB, SAB0846, SAB2176, SAB0566, SspA, and SspB forming the core exoproteome. Fifteen extracellular proteins were highly variably expressed and only present in less than 20% of the isolates. This includes the serine proteases SplB, C, and F, and the superantigens SEC-bov, SEL and TSST-1. Compared to human isolates we identified at least six proteins with significantly different expression frequencies. While SAB0846 was expressed more frequently in bovine isolates, LytM, EbpS, Spa, Geh, and LukL1 were seen less frequently in these isolates.
金黄色葡萄球菌不仅定植和感染人类，也会感染动物。在本研究中，我们对 17 株从患有乳腺炎的奶牛中分离出的金黄色葡萄球菌菌株进行了特征分析。我们采用了成熟的多位点序列分型（MLST）技术和一个涵盖 185 个金黄色葡萄球菌毒力和耐药基因的诊断微阵列，用于进行遗传和流行病学分析。通过分析每个分离株在 2-DE 凝胶上的细胞外蛋白图谱，进行了毒力基因表达研究。通过这种方式，我们观察到牛源分离株之间的细胞外蛋白质组存在明显的异质性，这归因于基因组的可塑性和基因表达的变化。仅有 12 种蛋白至少在 80%的分离株中表达，即 Atl、Aur、GlpQ、Hla、LtaS、Nuc、PdhB、SAB0846、SAB2176、SAB0566、SspA 和 SspB，形成了核心细胞外蛋白质组。15 种细胞外蛋白的表达高度可变，仅存在于不到 20%的分离株中。这包括丝氨酸蛋白酶 SplB、C 和 F，以及超抗原 SEC-bov、SEL 和 TSST-1。与人类分离株相比，我们至少鉴定出了 6 种表达频率有显著差异的蛋白。虽然 SAB0846 在牛源分离株中表达更为频繁，但 LytM、EbpS、Spa、Geh 和 LukL1 在这些分离株中表达频率较低。
