Morais Danielle B, Barros Mirlaine S, Freitas Mariella B D, Paula Tarcízio A R, Matta Sérgio L P
Department of Morphology, Federal University of Rio Grande do Norte, Natal-RN, Brazil.
Department of Animal Biology, Federal University of Viçosa, Viçosa-MG, Brazil.
Anim Reprod Sci. 2014 Jun 30;147(3-4):180-6. doi: 10.1016/j.anireprosci.2014.03.008. Epub 2014 Apr 5.
Due to the scarcity of information about the reproduction of bats, it is necessary to perform studies on different species to identify the occurrence of annual variations in their reproductive process. Therefore, the aims of this study were to describe and quantify the intertubular components within the testes of the bat Sturnira lilium and to verify whether seasonality takes place in spermatogenesis of this species. The animals were collected in different seasons and time of collection was grouped into dry and rainy seasons. Testicular fragments were routinely processed for light and transmission electron microscopies and blood samples from each animal were collected for quantification of plasma testosterone. Overall, the Leydig cells (LC) were the main intertubular component (83.2%), with abundant lipid droplets in their cytoplasm. Ultrastructural analysis indicated collagen fibers in the connective tissue and lymphatic spaces, with thin walls, surrounding the seminiferous tubules. The proportion and volume of each intertubular component did not vary significantly between seasons. On average, testosterone concentrations did not vary between rainy and dry seasons (21ng/mL). The total number of LC in the testis (50.0×10(5)) and per gram of testis (11×10(7)) did not vary nor did the Leydigosomatic Index (0.03%). Therefore, it is concluded that S. lilium had significant investment in intertubular tissues, especially in LC. Most of the variables that were assessed did not vary with season of the year, which leads to the assumption that S. lilium has a continuous reproductive cycle in southeastern Brazil.
由于关于蝙蝠繁殖的信息匮乏，有必要对不同物种进行研究，以确定其繁殖过程中年度变化的情况。因此，本研究的目的是描述和量化淡黄果蝠睾丸内的管间成分，并验证该物种的精子发生是否存在季节性。在不同季节收集动物，并将收集时间分为旱季和雨季。对睾丸组织切片进行常规处理，用于光镜和透射电镜观察，并采集每只动物的血液样本以定量血浆睾酮。总体而言，睾丸间质细胞（LC）是主要的管间成分（83.2%），其细胞质中含有丰富的脂滴。超微结构分析表明，结缔组织和淋巴间隙中有胶原纤维，其薄壁围绕着生精小管。各管间成分的比例和体积在不同季节之间没有显著差异。平均而言，雨季和旱季的睾酮浓度没有变化（21ng/mL）。睾丸中LC的总数（50.0×10⁵）和每克睾丸中的数量（11×10⁷）以及间质细胞指数（0.03%）均无变化。因此，可以得出结论，淡黄果蝠在管间组织尤其是在LC方面有显著投入。大多数评估变量不会随年份季节变化，这表明淡黄果蝠在巴西东南部有连续的繁殖周期。
