Southern Knights' Laboratory LLP, University of the Ryukyus, Okinawa, Japan.
Southern Knights' Laboratory LLP, University of the Ryukyus, Okinawa, Japan.
J Urol. 2014 Oct;192(4):1278-85. doi: 10.1016/j.juro.2014.04.092. Epub 2014 Apr 30.
The rostral pontine reticular formation has a strong inhibitory effect on micturition by facilitating lumbosacral glycinergic neurons. We assessed the influence of the rostral pontine reticular formation on the micturition reflex after noradrenaline injection in the medial frontal lobe. We also examined the relation between the medial frontal lobe and the rostral pontine reticular formation.
Continuous cystometry was performed in 28 female rats. After the interval between bladder contractions was shortened by noradrenaline injection in the medial frontal lobe we injected glutamate or flavoxate hydrochloride in the rostral pontine reticular formation or intravenously injected flavoxate or propiverine. The change in bladder activity was examined.
Noradrenaline injection in the medial frontal lobe shortened the interval between bladder contractions. In contrast to the bladder contraction interval before and after noradrenaline injection in the medial frontal lobe, the interval was prolonged after noradrenaline injection when glutamate or flavoxate was injected in the rostral pontine reticular formation, or flavoxate was injected intravenously. Noradrenaline injection in the medial frontal lobe plus intravenous propiverine injection also prolonged the interval compared to that after noradrenaline injection alone. However, the interval after noradrenaline injection in the medial frontal lobe plus intravenous injection of propiverine was shorter than that before noradrenaline injection only.
Medial frontal lobe neurons excited by noradrenaline may facilitate the micturition reflex via activation of inhibitory interneurons, which inhibit descending rostral pontine reticular formation neurons that innervate the lumbosacral glycinergic inhibitory neurons. Therefore, the mechanism of micturition reflex facilitation by the activation of medial frontal lobe neurons involves the rostral pontine reticular formation.
脑桥头端网状结构通过促进腰骶部甘氨酸能神经元对排尿产生强烈的抑制作用。我们评估了去甲肾上腺素注射到额内侧核后，脑桥头端网状结构对排尿反射的影响。我们还研究了额内侧核与脑桥头端网状结构之间的关系。
对 28 只雌性大鼠进行连续膀胱测压。在额内侧核注射去甲肾上腺素缩短膀胱收缩间隔后，我们在脑桥头端网状结构注射谷氨酸或盐酸黄酮哌酯，或静脉注射黄酮哌酯或普罗维林。检查膀胱活动的变化。
额内侧核注射去甲肾上腺素缩短了膀胱收缩间隔。与额内侧核注射去甲肾上腺素前后的膀胱收缩间隔相比，当脑桥头端网状结构注射谷氨酸或盐酸黄酮哌酯或静脉注射黄酮哌酯时，间隔延长。与单独注射去甲肾上腺素相比，额内侧核注射去甲肾上腺素加静脉注射普罗维林也延长了间隔。然而，与仅注射去甲肾上腺素相比，额内侧核注射去甲肾上腺素加静脉注射普罗维林后的间隔更短。
被去甲肾上腺素兴奋的额内侧核神经元可能通过激活抑制性中间神经元来促进排尿反射，抑制投射到支配腰骶部甘氨酸能抑制性神经元的脑桥头端网状结构神经元。因此，激活额内侧核神经元促进排尿反射的机制涉及脑桥头端网状结构。
