Sahbaz Ahmet, Isik Hatice, Aynioglu Oner, Gungorduk Kemal, Gun Banu Dogan
Zonguldak Bulent Ecevit University, School of Medicine, Department of Obstetrics and Gynecology, Kozlu, Zonguldak, Turkey.
Zonguldak Bulent Ecevit University, School of Medicine, Department of Obstetrics and Gynecology, Kozlu, Zonguldak, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2014 Jun;177:44-7. doi: 10.1016/j.ejogrb.2014.03.018. Epub 2014 Apr 13.
OBJECTIVE(S): Peritoneal adhesion is a serious problem that develops after most abdominopelvic surgeries. Allium sativum (garlic) has been used for centuries as both a nutrient and a traditional medicine. The anti-inflammatory, antibacterial, fibrinolytic, and wound-healing properties of garlic are well-recognized. We evaluated the effect of garlic on formation of postoperative adhesions in rats.
Thirty Wistar-Albino female rats weighing 200-250 g were randomly divided into three groups (10 rats/group). Group 1 rats received 5 ml/kg garlic oil intraperitoneally and no surgery was performed. The ceca of Group 2 rats (controls) were scraped, to trigger adhesion formation, and no treatment was given. In Group 3 rats, 5 ml/kg garlic oil was added to the peritoneal cavity immediately after the cecum was scraped. All animals were sacrificed 10 d after surgery and adhesions graded in terms of severity and histopathologic characteristics.
All animals tolerated the operations well. No adhesions were evident upon laparotomy of Group 1 animals. In Group 2 three rats had an adhesion grade 2 and seven rats had an adhesion of grade 3, whereas in Group 3 no adhesions were found in four rats, five rats had an adhesion grade of 1. Only one rat had a grade 2 adhesion. Macroscopic adhesions and mean adhesion scores of Group 3 were significantly lower than Group 2 (p<0.001). Histopathologic evaluation of the specimens also revealed a statistically significant differences in inflammation, fibrosis, and neovascularization scores between Group 2 and 3 (p=0.001, p=0.001, and p=0.011, respectively). Inflammation, fibrosis and vascularization scores in Group 3 were found significantly lower than Group 2.
The anti-inflammatory, antibacterial, fibrinolytic, antithrombotic, and wound-healing effects of garlic likely prevent formation of peritoneal adhesions in a rat model, and garlic may be effective and cheap when used to prevent such adhesions in humans.
腹膜粘连是大多数腹部盆腔手术后出现的一个严重问题。大蒜几个世纪以来一直被用作营养物和传统药物。大蒜的抗炎、抗菌、纤维蛋白溶解和伤口愈合特性已得到充分认可。我们评估了大蒜对大鼠术后粘连形成的影响。
将30只体重200 - 250克的Wistar - Albino雌性大鼠随机分为三组（每组10只）。第1组大鼠腹腔注射5毫升/千克大蒜油，未进行手术。第2组大鼠（对照组）的盲肠被刮擦以引发粘连形成，未给予治疗。第3组大鼠在盲肠刮擦后立即向腹腔内注入5毫升/千克大蒜油。所有动物在手术后10天处死，并根据粘连的严重程度和组织病理学特征进行分级。
所有动物对手术耐受性良好。第1组动物剖腹探查时未见粘连。第2组中，3只大鼠粘连分级为2级，7只大鼠粘连分级为3级，而第3组中，4只大鼠未发现粘连，5只大鼠粘连分级为1级，仅1只大鼠粘连分级为2级。第3组的宏观粘连和平均粘连评分显著低于第2组（p<0.001）。标本的组织病理学评估还显示，第2组和第3组在炎症、纤维化和新血管形成评分方面存在统计学显著差异（分别为p = 0.001、p = 0.001和p = 0.011）。第3组的炎症、纤维化和血管形成评分显著低于第2组。
大蒜的抗炎、抗菌、纤维蛋白溶解、抗血栓形成和伤口愈合作用可能预防大鼠模型中腹膜粘连的形成，并且大蒜用于预防人类此类粘连时可能有效且廉价。
