酸模叶蓼预防大鼠手术诱导的腹腔内粘连模型的效果。
Preventive effect of Rumex crispus L. on surgically induced intra-abdominal adhesion model in rats.
机构信息
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.
Laboratory Animals Breeding and Experimental Research Center, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.
出版信息
Daru. 2021 Jun;29(1):101-115. doi: 10.1007/s40199-021-00387-8. Epub 2021 Mar 29.
BACKGROUND
Rumex crispus L. (Polygonaceae), known as "Labada" in Turkey, was reported to be used for the treatment of gynecological diseases such as postpartum complications and infertility in folk medicine. Earlier studies on R. crispus have shown that leaf, fruit and root extracts have anti-inflammatory and antioxidant activities and are used for the treatment of tumors in the uterus. The hypothesis of this study is that R. crispus may generate potential anti-adhesive activity against complex factors such as inflammation, oxidation and fibrosis.
OBJECTIVES
We aimed to investigate the potential anti-adhesive activity of aqueous methanol extracts of leaves, fruits and roots of R. crispus.
METHODS
Abdominal adhesion model was performed in 72 female Wistar Albino rats. In the first step of the experiment, the rats were divided into six groups namely, Sham, Control, Reference and Experimental Groups (consisting of three sub-groups in which R. crispus leaf, fruit and root extracts were applied at 100 mg/kg dose). The test samples were administered once to the peritoneal cavity and the rats were sacrificied at the end of the 14th day. Root extract showed prominent activity, therefore this extract was subjected to fractionation to obtain 3 fractions (30-60-100% methanol fractions) by using vacuum-liquid chromatography. In the second stage, animals were divided into 6 groups as Sham, Control, Reference and Experimental Groups (R30, R60, R100 at 100 mg/kg dose). Adhesion scoring, tissue total antioxidant and oxidant levels, histopathological and immunohistochemical (TNF-α, IL-6 and IL-8) analyzes were performed.
RESULTS AND CONCLUSION
Adhesion scores, inflammatory cytokines and inflammation cells decreased by the application of R. crispus root extract. The fractions also showed similar anti-inflammatory effects, but R60 was found to be more effective in prevention of intra-abdominal adhesions and uterine fibrosis. R60 fraction, possessing potential bioactivity, was investigated in terms of phenolic composition by HPLC.
背景
皱叶酸模（Polygonaceae）在土耳其被称为“Labada”，据民间医学报道，其叶子、果实和根可用于治疗产后并发症和不孕等妇科疾病。早期对皱叶酸模的研究表明，叶、果和根提取物具有抗炎和抗氧化活性，可用于治疗子宫肿瘤。本研究的假设是，皱叶酸模可能会产生针对炎症、氧化和纤维化等复杂因素的潜在抗粘连活性。
目的
研究皱叶酸模叶、果和根的水甲醇提取物的潜在抗粘连活性。
方法
在 72 只雌性 Wistar 白化大鼠中进行腹部粘连模型。在实验的第一步，将大鼠分为 6 组，即假手术组、对照组、参比组和实验组（分为 3 个亚组，其中皱叶酸模叶、果和根提取物以 100 mg/kg 的剂量给药）。将测试样品一次性施用于腹腔，在第 14 天结束时处死大鼠。根提取物表现出突出的活性，因此通过真空液-液色谱法，用 30%-60%-100%甲醇对其进行分级，得到 3 个馏分。在第二阶段，动物分为 6 组，即假手术组、对照组、参比组和实验组（R30、R60、R100 以 100 mg/kg 的剂量）。进行粘连评分、组织总抗氧化和氧化水平、组织病理学和免疫组织化学（TNF-α、IL-6 和 IL-8）分析。
结果与结论
皱叶酸模根提取物的应用降低了粘连评分、炎症细胞因子和炎症细胞。各馏分也表现出相似的抗炎作用，但 R60 被发现更能有效预防腹腔内粘连和子宫纤维化。R60 馏分具有潜在的生物活性，通过 HPLC 对其酚类成分进行了研究。
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