在缺乏供体匹配淋巴细胞的情况下，造血祖细胞快速诱导耐受性。

Rapid tolerance induction by hematopoietic progenitor cells in the absence of donor-matched lymphoid cells.

作者信息

Domen Jos, Li Yongwu, Sun Lei, Simpson Pippa, Gandy Kimberly

机构信息

Section of Cardiac Surgery, Children's Mercy Hospital and Clinics, Kansas City MO64108, United States; Department of Pediatrics, University of Missouri Kansas City, Kansas City, MO, United States.

Section of Cardiac Surgery, Children's Mercy Hospital and Clinics, Kansas City MO64108, United States.

出版信息

Transpl Immunol. 2014 Aug;31(2):112-8. doi: 10.1016/j.trim.2014.04.001. Epub 2014 Apr 30.

DOI:10.1016/j.trim.2014.04.001

PMID:24794050

Abstract

BACKGROUND

Donor specific hematopoietic cell transplantation has long been recognized for its potential in tolerance induction for subsequently transplanted organs. We have recently published that co-administration of Myeloid Progenitor (MP) and third party Hematopoietic Stem Cells (HSC) can induce MP-specific tolerance for subsequently transplanted organs [1].

METHODS

Mice received an allogeneic HSC and third party MP transplantation simultaneous with placement of a MP-matched skin graft. Variants tested include time of graft placement, MP genotype and source of cells.

RESULTS

Using B10;B6-Rag2(-/-)Il2rg(-/-) mice, we demonstrate that specific tolerance can be induced by MP given simultaneous with the skin graft in the complete absence of MP-donor-matched lymphoid cells. Ex vivo expanded MP function as well as sorted cells in inducing tolerance. In addition we demonstrate that tolerance can be induced by MP in the context of autologous HSC transplantation.

CONCLUSIONS

Our results demonstrate that the previously observed expansion of organ donor matched Treg is not essential for tolerance, and that MP tolerance protocols can be envisioned in most clinical settings, including those involving deceased donor organs.

摘要