Goosen Wynand J, Miller Michele A, Chegou Novel N, Cooper David, Warren Robin M, van Helden Paul D, Parsons Sven D C
DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Molecular and Cellular Biology/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, P.O. Box 19063, Tygerberg 7505, South Africa.
Ezemvelo KZN Wildlife, P.O. Box 25, Mtubatuba 3935, South Africa.
Vet Immunol Immunopathol. 2014 Jul 15;160(1-2):133-8. doi: 10.1016/j.vetimm.2014.03.015. Epub 2014 Apr 4.
We assessed the use of Mycobacterium bovis-specific peptides for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer) by evaluating the agreement between the single intradermal comparative tuberculin test (SICTT), the Bovigam(®) EC (BEC) assay, the Bovigam(®) HP (BHP) assay and two assays utilizing the QuantiFERON(®) TB-Gold (in tube) system employing 20 h (mQFT20 assay) and 30 h (mQFT30 assay) whole blood incubation periods. Of 84 buffaloes, 45% were SICTT-positive, 48% were BEC-positive, 50% were BHP-positive, 37% were mQFT20-positive and 43% were mQFT30-positive. Agreement between the BEC and BHP Bovigam(®) assays was high (κ=0.86, 95% CI 0.75-0.97) and these detected the most test-positive animals suggesting that they were the most sensitive assays. Interferon-gamma release was significantly greater in buffaloes that were test-positive for all tests than in animals with discordant but positive Bovigam(®) results. Agreement between the mQFT assays was equally high (κ=0.88, 95% CI 0.77-0.98); however, all buffaloes with discordant mQFT results (n=6) were mQFT30-positive/mQFT20-negative, including three confirmed M. bovis-infected animals, suggesting that the mQFT30 assay is the more sensitive of the two. Agreements between the two Bovigam(®) and two mQFT assays were moderate, suggesting that in its current format the mQFT assay is less sensitive than either the BEC or the BHP assays.
我们通过评估单剂量皮内比较结核菌素试验（SICTT）、Bovigam® EC（BEC）检测、Bovigam® HP（BHP）检测以及两种采用QuantiFERON® TB-Gold（试管内）系统且全血孵育时间分别为20小时（mQFT20检测）和30小时（mQFT30检测）的检测方法之间的一致性，来评估牛分枝杆菌特异性肽在非洲水牛（非洲野水牛）结核病诊断中的应用。在84头水牛中，45%的水牛SICTT检测呈阳性，48%的水牛BEC检测呈阳性，50%的水牛BHP检测呈阳性，37%的水牛mQFT20检测呈阳性，43%的水牛mQFT30检测呈阳性。BEC和BHP Bovigam®检测之间的一致性较高（κ = 0.86，95%置信区间0.75 - 0.97），并且这些检测方法检测出的检测阳性动物最多，这表明它们是最敏感的检测方法。在所有检测中呈阳性的水牛中，γ干扰素释放量显著高于Bovigam®检测结果不一致但呈阳性的动物。mQFT检测之间的一致性同样较高（κ = 0.88，95%置信区间0.77 - 0.98）；然而，所有mQFT检测结果不一致的水牛（n = 6）均为mQFT30阳性/mQFT20阴性，其中包括三头确诊感染牛分枝杆菌的动物，这表明mQFT30检测是两者中更敏感的检测方法。两种Bovigam®检测和两种mQFT检测之间的一致性为中等，这表明以其当前形式，mQFT检测不如BEC或BHP检测敏感。
