Yamamoto D, Suzuki N
Laboratory of Neurophysiology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Biochim Biophys Acta. 1989 Nov 17;986(1):187-90. doi: 10.1016/0005-2736(89)90289-7.
An anion channel from Drosophila neurons had the selectivity sequence: NO3- (1.97) greater than Br- (1.12) approximately equal to I- (1.03) approximately equal to Cl- (1) greater than F- (0.32) much greater than glutamate (less than 0.02) as estimated by the permeability ratio based on the reversal potential measurement. 4-Acetoamido-4'-isothiocyanostilbene-2,2'-disulfonic acid applied internally at 10 microM blocked the channel. We suggest that the chloride channel identified here may provide a pathway for Cl- in the resting membrane of Drosophila neurons.
通过基于反转电位测量的渗透率比值估计，果蝇神经元中的一种阴离子通道具有以下选择性序列：硝酸根离子（1.97）＞溴离子（1.12）≈碘离子（1.03）≈氯离子（1）＞氟离子（0.32）＞＞谷氨酸根离子（＜0.02）。内部施加10微摩尔的4-乙酰氨基-4'-异硫氰基芪-2,2'-二磺酸可阻断该通道。我们认为，此处鉴定出的氯离子通道可能为果蝇神经元静息膜中的氯离子提供一条途径。
