聚乙二醇诱导的葡聚糖凝胶收缩。用于渗透弹性耦合定量分析的模型系统。

Poly(ethylene glycol)-induced shrinkage of Sephadex gel. A model system for quantitative analysis of osmoelastic coupling.

作者信息

Ito T, Yamazaki M, Ohnishi S

机构信息

Department of Biophysics, Faculty of Science, Kyoto University, Japan.

出版信息

Biophys J. 1989 Oct;56(4):707-11. doi: 10.1016/S0006-3495(89)82717-1.

DOI:10.1016/S0006-3495(89)82717-1

PMID:2479421

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1280526/

Abstract

Shrinkage of Sephadex gels caused by addition of a high-molecular weight molecule, poly(ethylene glycol) (PEG) was studied. A quantitative analysis based on the cross-linked network theory by Flory and Tanaka (Tanaka, T. 1978. Phys. Rev. Lett. 40:820-823) showed that the shrinkage is due to a mechanochemical coupling between the elasticity of the network and the osmotic stress arising from preferential exclusion of PEG. These results may provide good evidence for "osmoelastic coupling", the coupling between elasticity of macromolecular structures and osmotic stress, which has been predicted in some biological systems such as phospholipid bilayer membranes (Ito, T., M. Yamazaki, and S. Ohnishi. 1989. Biochemistry. 28:5626-5630; Yamazaki, M., S. Ohnishi, and T. Ito. 1989. Biochemistry. 28:3710-3715) or actin filaments.

摘要

研究了添加高分子量分子聚乙二醇（PEG）导致的葡聚糖凝胶收缩现象。基于Flory和Tanaka的交联网络理论（Tanaka, T. 1978. Phys. Rev. Lett. 40:820 - 823）进行的定量分析表明，收缩是由于网络弹性与PEG优先排除所产生的渗透应力之间的机械化学耦合。这些结果可能为“渗透弹性耦合”提供有力证据，“渗透弹性耦合”是指大分子结构弹性与渗透应力之间的耦合，在一些生物系统如磷脂双分子层膜（Ito, T., M. Yamazaki, and S. Ohnishi. 1989. Biochemistry. 28:5626 - 5630; Yamazaki, M., S. Ohnishi, and T. Ito. 1989. Biochemistry. 28:3710 - 3715）或肌动蛋白丝中已被预测。