胸膜肺炎放线杆菌血清型4（ATCC 33378）产生的脂多糖抗原O链的结构

Structure of the lipopolysaccharide antigenic O-chain produced by Actinobacillus pleuropneumoniae serotype 4 (ATCC 33 378).

作者信息

Altman E, Perry M B, Brisson J R

机构信息

Division of Biological Sciences, National Research Council of Canada, Ottawa.

出版信息

Carbohydr Res. 1989 Aug 15;191(2):295-303. doi: 10.1016/0008-6215(89)85072-4.

DOI:10.1016/0008-6215(89)85072-4

PMID:2479478

Abstract

The structure of the antigenic O-polysaccharide part of the S-type lipopolysaccharide produced by Actinobacillus pleuropneumoniae serotype 4 has been determined by periodate oxidation, methylation, partial hydrolysis, and 1H- and 13C-n.m.r. spectroscopy. The O-polysaccharide structure has a branched-tetrasaccharide repeating unit, (----3)-beta-D-Galp-(1----4)-[beta-D-Glcp-(1----3)]-beta-D-GalpNAc- (1----4)-alpha-L-Rhap-(1-)n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7, and their common epitopes may account for the apparent serological cross-reactivity observed between the two serotypes when incompletely adsorbed, anticapsular-typing sera are used.

摘要

通过高碘酸盐氧化、甲基化、部分水解以及1H-和13C-核磁共振光谱法，已确定胸膜肺炎放线杆菌4型产生的S型脂多糖的抗原性O-多糖部分的结构。O-多糖结构具有一个分支四糖重复单元，(----3)-β-D-吡喃半乳糖-(1----4)-[β-D-吡喃葡萄糖-(1----3)]-β-D-吡喃半乳糖胺-(1----4)-α-L-鼠李糖-(1-)n。该结构类似于胸膜肺炎放线杆菌7型脂多糖O链的结构，当使用未完全吸附的抗荚膜分型血清时，它们的共同表位可能解释了在这两种血清型之间观察到的明显血清学交叉反应性。