洋葱伯克霍尔德菌A血清型脂多糖O抗原的结构

Structure of the lipopolysaccharide O-antigen of Pseudomonas cepacia serotype A.

作者信息

Beynon L M, Perry M B

机构信息

Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada.

出版信息

Biochem Cell Biol. 1993 Sep-Oct;71(9-10):417-20. doi: 10.1139/o93-061.

DOI:10.1139/o93-061

PMID:7514883

Abstract

The O-antigenic polysaccharides of the lipopolysaccharides produced by three isolates of Pseudomonas cepacia serogroup A were analysed by composition, methylation, periodate oxidation, and two-dimensional nuclear magnetic resonance methods. They were found to be identical linear unbranched polymers of a repeating trisaccharide unit containing 2-acetamido-2-deoxy-D-galactose and L-rhamnose residues (2:1) and have the structure [-->3)-alpha-D-GalpNAc-(1-->3)-beta-D-GalpNAc-(1-->4)-alpha-L-Rhap -(1-->]n.

摘要

通过组成分析、甲基化、高碘酸盐氧化和二维核磁共振方法，对洋葱伯克霍尔德菌血清群A的三株分离株产生的脂多糖的O抗原多糖进行了分析。发现它们是重复三糖单元的相同线性无分支聚合物，该三糖单元含有2-乙酰氨基-2-脱氧-D-半乳糖和L-鼠李糖残基（2:1），其结构为[-->3)-α-D-半乳糖胺-(1-->3)-β-D-半乳糖胺-(1-->4)-α-L-鼠李糖-(1-->]n。