Petrocelli Iacopo, Turillazzi Stefano, Delfino Giovanni
Università degli Studi di Firenze, Dipartimento di Biologia, Via Madonna del Piano 6, 50019, Sesto Fiorentino, Firenze, Italy.
Università degli Studi di Firenze, Dipartimento di Biologia, Via Madonna del Piano 6, 50019, Sesto Fiorentino, Firenze, Italy.
Arthropod Struct Dev. 2014 Sep;43(5):457-68. doi: 10.1016/j.asd.2014.04.007. Epub 2014 May 4.
In the wasp venom apparatus, the convoluted gland is the tract of the thin secretory unit, i.e. filament, contained in the muscular reservoir. Previous transmission electron microscope investigation on Stenogastrinae disclosed that the free filaments consist of distal and proximal tracts, from/to the venom reservoir, characterized by class 3 and 2 gland patterns, respectively. This study aims to extend the ultrastructural analysis to the convoluted tract, in order to provide a thorough, subcellular representation of the venom gland in these Asian wasps. Our findings showed that the convoluted gland is a continuation of the proximal tract, with secretory cells provided with a peculiar apical invagination, the extracellular cavity, collecting their products. This compartment holds a simple end-apparatus lined by large and ramified microvilli that contribute to the processing of the secretory product. A comparison between previous and present findings reveals a noticeable regionalization of the stenogastrine venom filaments and suggests that the secretory product acquires its ultimate composition in the convoluted tract.
在黄蜂的毒液器官中，蟠曲腺是包含在肌肉贮液囊内的细分泌单位（即细丝）的通道。先前对狭腹胡蜂亚科进行的透射电子显微镜研究表明，游离细丝由分别来自/通向毒液贮液囊的远端和近端通道组成，其特征分别为3类和2类腺体模式。本研究旨在将超微结构分析扩展至蟠曲通道，以便对这些亚洲黄蜂的毒腺进行全面的亚细胞层面描述。我们的研究结果表明，蟠曲腺是近端通道的延续，分泌细胞具有特殊的顶端内陷（即细胞外腔）来收集其产物。这个腔室有一个由大的分支微绒毛排列而成的简单终末装置，有助于分泌产物的加工。对先前和当前研究结果的比较揭示了狭腹胡蜂毒液细丝明显的区域化，并表明分泌产物在蟠曲通道中获得其最终成分。
