Virology Department, Pasteur Institute of Iran, Tehran, Iran; Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Med Virol. 2015 Jan;87(1):102-11. doi: 10.1002/jmv.23973. Epub 2014 May 5.
In this study, stable high-five insect cell line constitutively expressing rotavirus (RV) VP2 was co-transfected with VP6 and VP7-recombinant plasmids. The presence of RV proteins in stably transfected high-five cells was verified by molecular and protein analyses. To yield self-assembled triple-layered RV-like particles (tlRLPs), a stable insect high-five cell line was generated to produce RV VP6 and VP7 besides VP2. Self-assembled tlRLPs were observed by transmission electron microscopy (TEM), and enzyme-linked immunosorbent assay (ELISA) was used to assess their antigenicity in vivo. The results suggest that the stable transfected high-five cells are able to generate tlRLPs with the efficient antigenicity.
在这项研究中，稳定高表达五细胞系持续表达轮状病毒（RV）VP2 与 VP6 和 VP7-重组质粒共转染。RV 蛋白的存在通过分子和蛋白质分析证实稳定转染高五细胞。产生自组装三层轮状病毒样颗粒（tlRLPs），一种稳定的昆虫高五细胞系产生 RV VP6 和 VP7 除 VP2。自组装 tlRLPs 通过透射电子显微镜（TEM）观察，并通过酶联免疫吸附试验（ELISA）评估其体内抗原性。结果表明，稳定转染的高五细胞系能够产生具有高效抗原性的 tlRLPs。
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