利用杆状病毒表达系统生产A组和C组轮状病毒的杂交双层或三层病毒样颗粒。

Production of hybrid double- or triple-layered virus-like particles of group A and C rotaviruses using a baculovirus expression system.

作者信息

Kim Yunjeong, Chang Kyeong Ok, Kim Won Yong, Saif Linda J

机构信息

Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691, USA.

出版信息

Virology. 2002 Oct 10;302(1):1-8. doi: 10.1006/viro.2002.1610.

DOI:10.1006/viro.2002.1610

PMID:12429511

Abstract

Dual infections by group A and group C rotaviruses have been reported, but no reassortants between group A and group C rotaviruses have been described. The VP6 major inner capsid protein of group A and C rotaviruses shares common antigens detected by monoclonal antibodies and also shares 40-43% amino acid identity. Coinfection of Spodoptera frugiperda (Sf9) insect cells with different combinations of the recombinant baculoviruses encoding either group A [RF VP2 (A-VP2), IND VP6 (A-VP6), and VP7 (A-VP7[IND]), 2292B VP7 (A-VP7[2292B])] or C [Shintoku VP6 (C-VP6) and VP7 (C-VP7)] bovine rotavirus proteins produced hybrid group A/C triple-layered VP2/6/7 virus-like particles (TLPs) composed of A-VP2/C-VP6/C-VP7, A-VP2/C-VP6/A-VP7(IND), A-VP2/C-VP6/A-VP7(2292B), and A-VP2/A-VP6/C-VP7. To our knowledge, this is the first report that the inner capsid VP6 of group A or group C rotavirus can support attachment of the heterologous, antigenically distinct group A (G6, IND or G10, 2292B) or group C rotavirus outer capsid VP7 to produce hybrid TLPs in vitro.

摘要

已有A组和C组轮状病毒双重感染的报道，但尚未描述A组和C组轮状病毒之间的重配体。A组和C组轮状病毒的VP6主要内衣壳蛋白具有单克隆抗体检测到的共同抗原，氨基酸同源性也有40%-43%。用编码A组[RF VP2（A-VP2）、IND VP6（A-VP6）和VP7（A-VP7[IND]）、2292B VP7（A-VP7[2292B]）]或C组[新德VP6（C-VP6）和VP7（C-VP7）]牛轮状病毒蛋白的重组杆状病毒的不同组合共同感染草地贪夜蛾（Sf9）昆虫细胞，产生了由A-VP2/C-VP6/C-VP7、A-VP2/C-VP6/A-VP7（IND）、A-VP2/C-VP6/A-VP7（2292B）和A-VP2/A-VP6/C-VP7组成的杂交A/C组三层VP2/6/7病毒样颗粒（TLP）。据我们所知，这是首次报道A组或C组轮状病毒的内衣壳VP6能够支持异源的、抗原性不同的A组（G6、IND或G10、2292B）或C组轮状病毒外衣壳VP7的附着，从而在体外产生杂交TLP。