Kajigaya Y, Sasaki H, Ikuta K, Matsuyama S
Nihon Ketsueki Gakkai Zasshi. 1989 Aug;52(5):835-41.
We tested purified recombinant hemopoietic factors for their effects on the proliferation and differentiation of murine myelomonocytic leukemia cells (WEHI-3B-Y1) in serum-free agar culture. We found that purified recombinant human G colony-stimulating factor (CSF) markedly increased the colony number of WEHI-3B-Y1 cells and differentiation-inducing activity. However, at low colony densities [( 100/dish), G-CSF did not induce the differentiation of WEHI-3B-Y1 cells. We conclude that G-CSF does not induce the differentiation of WEHI-3B-Y1 cells directly, but induce the differentiation as a result of the secondary autoinduction of differentiation. Interleukin 3 (IL-3) slightly enhanced but erythropoietin (Epo) did not alter the colony number of WEHI-3B-Y1 cells. GM-CSF or M-CSF decreased the colony number of WEHI-3B-Y1 cells. Such purified recombinant human IL-3, Epo, GM-CSF and M-CSF did not induce morphologically the distinct differentiation of WEHI-3B-Y1 cells.
我们在无血清琼脂培养中测试了纯化的重组造血因子对鼠骨髓单核细胞白血病细胞（WEHI-3B-Y1）增殖和分化的影响。我们发现纯化的重组人粒细胞集落刺激因子（CSF）显著增加了WEHI-3B-Y1细胞的集落数量和诱导分化活性。然而，在低集落密度[（100/培养皿）]时，G-CSF并未诱导WEHI-3B-Y1细胞的分化。我们得出结论，G-CSF并非直接诱导WEHI-3B-Y1细胞的分化，而是通过分化的二次自诱导作用来诱导分化。白细胞介素3（IL-3）略有增强，但促红细胞生成素（Epo）并未改变WEHI-3B-Y1细胞的集落数量。粒细胞巨噬细胞集落刺激因子（GM-CSF）或巨噬细胞集落刺激因子（M-CSF）减少了WEHI-3B-Y1细胞的集落数量。这种纯化的重组人IL-3、Epo、GM-CSF和M-CSF并未在形态上诱导WEHI-3B-Y1细胞发生明显分化。
