Wolfs T F, Geelen J L, Schellekens H, Barin F, Dekker J T, Goudsmit J
Department of Virology, Academic Medical Centre, University of Amsterdam, The Netherlands.
AIDS Res Hum Retroviruses. 1989 Oct;5(5):535-40. doi: 10.1089/aid.1989.5.535.
The inhibition of HIV-1 and SIV reverse transcriptase by human and rhesus macaque serum positive for HIV-1 or HIV-2/SIV antibodies was studied. The domain to which reverse transcriptase-inhibiting antibodies were elicited appeared to be highly antigenic. A total of 67% (48 of 72) of individuals had HIV-1 reverse transcriptase-inhibiting (RTI) antibodies 1 year after seroconversion for HIV-1, 90% (9 of 10) of HIV-2 antibody positive persons had SIV RTI antibodies, and all four experimentally SIV-infected rhesus macaques developed SIV RTI antibodies. Low cross-reactivity between HIV-1 and HIV-2/SIV RTI antibodies was observed. Of 10 HIV-1 RTI sera, 2 reduced SIV RT activity by more than 50% (mean reduction 85 versus 24%). Only 1 of 9 SIV RTI human sera reduced HIV-1 RT (mean reduction 74 versus 25%). This serum, however, showed a shared reactivity against both HIV-1 and HIV-2. These results indicate that the HIV-1 domain inducing RTI antibodies is antigenically different from the HIV-2/SIV domain.
对HIV-1或HIV-2/SIV抗体呈阳性的人血清和恒河猴血清对HIV-1和SIV逆转录酶的抑制作用进行了研究。引发逆转录酶抑制抗体的结构域似乎具有高度抗原性。共有67%（72例中的48例）个体在HIV-1血清转换1年后产生了HIV-1逆转录酶抑制（RTI）抗体，90%（10例中的9例）HIV-2抗体阳性者具有SIV RTI抗体，并且所有4只实验性感染SIV的恒河猴都产生了SIV RTI抗体。观察到HIV-1和HIV-2/SIV RTI抗体之间的交叉反应性较低。在10份HIV-1 RTI血清中，2份使SIV RT活性降低超过50%（平均降低85%对24%）。9份SIV RTI人血清中只有1份降低了HIV-1 RT（平均降低74%对25%）。然而，该血清对HIV-1和HIV-2均表现出共同反应性。这些结果表明，诱导RTI抗体的HIV-1结构域在抗原性上不同于HIV-2/SIV结构域。
