Nai Yu-Shin, Su Ping-Yi, Hsu Yu-Hsiang, Chiang Ching-Hao, Kim Jae Su, Chen Yue-Wen, Wang Chung-Hsiung
Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC; Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea.
Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC.
J Invertebr Pathol. 2014 Jul;120:4-8. doi: 10.1016/j.jip.2014.04.006. Epub 2014 May 4.
We briefly described the morphology and transmission pathway of a Spiroplasma sp. isolated from the field cricket, Gryllus bimaculatus in Taiwan, followed by the phylogenetic analysis based on the 16S rRNA gene sequence. The cricket spiroplasma infected the hemolymph, gut, muscle tissues and tracheal cells; therefore we suggest that the pathogen invaded tissues and organs from the hemolymph through the tracheal system and the endoplasmic reticular system. Based on 16S rRNA gene sequences and the phylogeny, this spiroplasma was most closely related to Spiroplasma platyhelix (Identity=95%) isolated from the dragonfly Pachydiplax longipennis and belongs to the Ixodetis clade.
我们简要描述了从台湾双斑蟋蟀体内分离出的一种螺旋体属物种的形态和传播途径，随后基于16S rRNA基因序列进行了系统发育分析。蟋蟀螺旋体感染血淋巴、肠道、肌肉组织和气管细胞；因此我们认为该病原体从血淋巴通过气管系统和内质网系统侵入组织和器官。基于16S rRNA基因序列和系统发育关系，这种螺旋体与从长尾蜓 Pachydiplax longipennis 分离出的扁平螺旋体（同一性 = 95%）关系最为密切，属于硬蜱分支。
