Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Research Center for Environmental Genomics, Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
FEBS Lett. 2014 May 29;588(11):2025-30. doi: 10.1016/j.febslet.2014.04.032. Epub 2014 May 5.
The MEKK1-MKK2-MPK4 cascade is activated during cold acclimation. However, little is known regarding the perception of low temperature. In this study, we demonstrate that treatment of Arabidopsis with a membrane rigidifier, DMSO, caused MPK4 activation concomitantly with MEKK1 and MKK2 phosphorylation, as well as the cold-inducible gene COR15a expression. These processes are similar to the effects of cold treatment, whereas benzyl alcohol (BA), a membrane fluidizer, prevented such cold-induced events. Moreover, the DMSO-treated seedlings acquired freezing tolerance without cold acclimation. In contrast, the BA-pretreated seedlings did not show freezing tolerance. These results suggest that membrane rigidification activates this MAPK cascade and contributes to the acquisition of freezing tolerance.
MEKK1-MKK2-MPK4 级联在冷驯化过程中被激活。然而，对于低温的感知知之甚少。在这项研究中，我们证明了用膜刚性剂 DMSO 处理拟南芥会导致 MPK4 激活，同时伴随着 MEKK1 和 MKK2 的磷酸化，以及冷诱导基因 COR15a 的表达。这些过程类似于低温处理的效果，而膜流动性促进剂苯甲醇（BA）则阻止了这种冷诱导事件的发生。此外，用 DMSO 处理的幼苗在未经冷驯化的情况下获得了抗冻性。相比之下，用 BA 预处理的幼苗则没有表现出抗冻性。这些结果表明，膜刚性化激活了这种 MAPK 级联反应，并有助于获得抗冻性。
