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[采用双重活细胞染色法研究特定刺激物对悬浮角质形成细胞的影响]

[The effect of selected irritants on suspended keratinocytes using double vital staining].

作者信息

Heise P, Heise H, Wohlrab W

出版信息

Dermatol Monatsschr. 1989;175(10):647-54.

PMID:2480254
Abstract

Loss of vitality of keratinocytes after influence of strong allergens and irritants was examined. Cell viability was determined by trypan blue exclusion assay and eosintyrode solution. The results of the two methods harmonize essentially, but obviously, single noxa can lead to changes of the dye that exclude the use for an appropriate test. On principle, the method is suitable for testing the substances. The toxic final concentration should constantly be ascertained. Still, the results are recommended to be guaranteed through other methods.

摘要

研究了强过敏原和刺激物作用后角质形成细胞活力的丧失情况。通过台盼蓝排斥试验和伊红-台盼蓝溶液测定细胞活力。两种方法的结果基本一致,但显然,单一损伤因素可导致染料变化,从而无法用于适当的检测。原则上,该方法适用于检测这些物质。应始终确定有毒最终浓度。尽管如此,仍建议通过其他方法来确保结果的可靠性。

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