Center of Excellence for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, 20500 Turku, Finland.
Division of Pharmaceutical Biosciences, Centre for Drug Research, Laboratory of Biopharmaceutics, Faculty of Pharmacy, 00014 University of Helsinki, Finland.
Colloids Surf B Biointerfaces. 2014 Jun 1;118:261-9. doi: 10.1016/j.colsurfb.2014.03.050. Epub 2014 Apr 18.
Specific interactions of extracellular matrix proteins with cells and their adhesion to the substrate are important for cell growth. A nanopatterned latex-coated paper substrate previously shown to be an excellent substrate for cell adhesion and 2D growth was studied for directed immobilization of proteins. The nanostructured latex surface was formed by short-wavelength IR irradiation of a two-component latex coating consisting of a hydrophilic film-forming styrene butadiene acrylonitrile copolymer and hydrophobic polystyrene particles. The hydrophobic regions of the IR-treated latex coating showed strong adhesion of bovine serum albumin (cell repelling protein), fibronectin (cell adhesive protein) and streptavidin. Opposite to the IR-treated surface, fibronectin and streptavidin had a poor affinity toward the untreated pristine latex coating. Detailed characterization of the physicochemical surface properties of the latex-coated substrates revealed that the observed differences in protein affinity were mainly due to the presence or absence of the protein repelling polar and charged surface groups. The protein adsorption was assisted by hydrophobic (dehydration) interactions.
细胞外基质蛋白与细胞的特定相互作用及其对底物的黏附对于细胞生长很重要。先前已经证明，经过纳米图案化的乳胶涂层纸基底非常适合细胞黏附和 2D 生长，可用于蛋白质的定向固定。通过对由亲水性成膜苯乙烯-丁二烯-丙烯腈共聚物和疏水性聚苯乙烯颗粒组成的双组分乳胶涂层进行短波长红外辐射，形成了纳米结构的乳胶表面。经 IR 处理的乳胶涂层的疏水区表现出牛血清白蛋白（细胞排斥蛋白）、纤连蛋白（细胞黏附蛋白）和链霉亲和素的强黏附性。与 IR 处理表面相反，纤连蛋白和链霉亲和素与未经处理的原始乳胶涂层的亲和力很差。对乳胶涂层基底的物理化学表面特性的详细表征表明，观察到的蛋白质亲和力差异主要归因于存在或不存在排斥极性和带电表面基团。蛋白质吸附是通过疏水性（脱水）相互作用来辅助的。
