一种无需设备的聚二甲基硅氧烷微流控点样器，用于制造微阵列。

An equipment-free polydimethylsiloxane microfluidic spotter for fabrication of microarrays.

机构信息

State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China ; Graduate University of Chinese Academy of Sciences, Beijing 100039, China.

State Key Lab of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

出版信息

Biomicrofluidics. 2014 Apr 17;8(2):026501. doi: 10.1063/1.4871935. eCollection 2014 Mar.

DOI:10.1063/1.4871935

PMID:24803969

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4000390/

Abstract

This paper presents a low-cost, power-free, and easy-to-use spotter system for fabrication of microarrays. The spotter system uses embedded dispensing microchannels combined with a polydimethylsiloxane (PDMS) membrane containing regular arrays of well-defined thru-holes to produce precise, uniform DNA or protein microarrays for disease diagnosis or drug screening. Powered by pre-evacuation of its PDMS substrate, the spotter system does not require any additional components or external equipment for its operation, which can potentially allow low-cost, high-quality microarray fabrication by minimally trained individuals. Polyvinylpyrrolidone was used to modify the PDMS surface to prevent protein adsorption by the microchannels. Experimental results indicate that the PDMS spotter shows excellent printing performance for immobilizing proteins. The measured coefficient of variation (CV) of the diameter of 48 spots was 2.63% and that of the intensity within one array was 2.87%. Concentration gradient experiments revealed the superiority of the immobilization density of the PDMS spotter over the conventional pin-printing method. Overall, this low-cost, power-free, and easy-to-use spotting system provides an attractive new method to fabricate microarrays.

摘要

本文提出了一种低成本、无电源、易于使用的点样系统，用于微阵列的制备。该点样系统使用嵌入式分配微通道与含有规则排列的通孔的聚二甲基硅氧烷 (PDMS) 膜结合，以产生用于疾病诊断或药物筛选的精确、均匀的 DNA 或蛋白质微阵列。该点样系统通过 PDMS 基板的预抽空来提供动力，无需任何额外的组件或外部设备即可运行，这可能允许经过最少培训的人员进行低成本、高质量的微阵列制造。聚乙烯吡咯烷酮被用于修饰 PDMS 表面，以防止蛋白质在微通道中的吸附。实验结果表明，PDMS 点样器在固定蛋白质方面表现出优异的打印性能。48 个点的直径的测量变异系数 (CV) 为 2.63%，一个阵列内的强度的 CV 为 2.87%。浓度梯度实验揭示了 PDMS 点样器的固定化密度优于传统的针印法。总的来说，这种低成本、无电源、易于使用的点样系统为微阵列的制备提供了一种有吸引力的新方法。