Bedini J L, Casals E, Sanllehy C, Filella X, Mas E, Molina R, Ballesta A M
J Nucl Med Allied Sci. 1989 Jul-Sep;33(3 Suppl):135-7.
We have evaluated the analytic performance of a new immunonephelometric method developed for the measurement of amniotic fluid alpha-fetoprotein (AF-AFP) and compared it with the classic immunoradiometric method used up to now. Intrassay precision, evaluated for three AF-AFP levels, was good and Interassay imprecision shows higher coefficients of variation (CVs) especially for medium and high AF-AFP levels. Linearity was demonstrated for level higher than 300,000 ng/ml and no hook effects was observed. Comparison with an IRMA method gave the following regression equation: y = 3050 + 0.52X (r = 0.76, p less than 0.001, n = 36).
我们评估了一种新开发的用于测量羊水甲胎蛋白（AF-AFP）的免疫比浊法的分析性能，并将其与目前使用的经典免疫放射分析法进行了比较。对三个AF-AFP水平评估的批内精密度良好，批间不精密度显示出较高的变异系数（CV），尤其是对于中高AF-AFP水平。对于高于300,000 ng/ml的水平证明了线性，并且未观察到钩状效应。与免疫放射分析法（IRMA）的比较给出了以下回归方程：y = 3050 + 0.52X（r = 0.76，p小于0.001，n = 36）。
