J Biomed Nanotechnol. 2014 Jul;10(7):1382-90. doi: 10.1166/jbn.2014.1834.
The combination of iontophoresis with solid lipid nanoparticles (SLNs) for targeting drug delivery to the epidermis has not been explored. The goal of this paper was to study the influence of iontophoresis on the penetration of doxorubicin (DOX) delivered in SLNs (DOX-SLNs). We measured the contribution of electroosmotic flow to the transport of DOX, and the accumulation of DOX in the stratum corneum (SC) and in the viable epidermis was determined. In addition, we evaluated the cytotoxicity of DOX-SLNs against skin cancer cells. Iontophoresis of unloaded SLNs decreased the electroosmotic flow by a factor of 5 and increased the skin resistance. Nevertheless, iontophoresis of DOX-SLNs increased DOX delivery to the viable epidermis, with 56% of all DOX penetrating this skin layer. Only 26% of the drug was retained in the SC. In contrast, passive delivery retained 43% of DOX in the SC and 26% in the viable epidermis. DOX-SLNs increased DOX cytotoxicity against melanoma cells by 50%. These results suggest the use of DOX-SLN iontophoresis in the topical treatment of skin cancer.
电渗析与固体脂质纳米粒（SLNs）联合用于将药物靶向递送至表皮尚未得到探索。本文的目的是研究电渗析对包载于固体脂质纳米粒中的阿霉素（DOX）渗透的影响。我们测量了电渗流对 DOX 传递的贡献，并测定了 DOX 在角质层（SC）和有活力的表皮中的积累。此外，我们评估了 DOX-SLNs 对皮肤癌细胞的细胞毒性。未载药的 SLNs 的电渗析使电渗流减少了 5 倍，并增加了皮肤电阻。然而，DOX-SLNs 的电渗析增加了 DOX 向有活力的表皮的传递，有 56%的 DOX 穿透了这一皮肤层。只有 26%的药物保留在 SC 中。相比之下，被动传递将 43%的 DOX 保留在 SC 中，将 26%的 DOX 保留在有活力的表皮中。DOX-SLNs 使 DOX 对黑色素瘤细胞的细胞毒性增加了 50%。这些结果表明，DOX-SLN 电渗析可用于皮肤癌的局部治疗。
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