Krenz B, Thompson J R, McLane H L, Fuchs M, Perry K L
Phytopathology. 2014 Nov;104(11):1232-40. doi: 10.1094/PHYTO-02-14-0053-R.
Grapevine red blotch disease has been recognized since 2008 as affecting North American grape production. The presence of the newly described Grapevine red blotch-associated virus (GRBaV) is highly correlated with the disease. To more effectively detect and monitor the presence of the virus, a sample processing strategy and multiplex polymerase chain reaction assay were developed. A total of 42 of 113 vine samples collected in or received from seven of the United States were shown to harbor the virus, demonstrating the virus is widely distributed across North America. Phylogenetic analyses of a viral replication-associated protein (Rep) gene fragment from the 42 isolates of GRBaV demonstrated distinct clades of the virus (1 and 2), with clade 1 showing the greatest variability. The full-length genome of six virus isolates was sequenced, and phylogenetic analyses of 14 whole genomes recapitulated results seen for the Rep gene. A comparison of GRBaV genomes revealed evidence of recombination underlying some of the variation seen among GRBaV genomes within clade 1. Phylogenetic analyses of coat and replicase-associated protein sequences among single-stranded DNA viruses showed GRBaV to group within the family Geminiviridae. This grouping is distinct from members of the families Nanoviridae and Circoviridae, with limited significant affinities to both recognized genera and novel plant-infecting, gemini-like viruses.
葡萄红斑点病自2008年以来被认为影响北美葡萄生产。新描述的葡萄红斑点相关病毒（GRBaV）的存在与该病高度相关。为了更有效地检测和监测该病毒的存在，开发了一种样品处理策略和多重聚合酶链反应检测方法。在美国七个州采集或收到的113个葡萄藤样品中，共有42个被证明含有该病毒，这表明该病毒在北美广泛分布。对来自42个GRBaV分离株的病毒复制相关蛋白（Rep）基因片段进行系统发育分析，显示该病毒有不同的进化枝（1和2），其中进化枝1表现出最大的变异性。对六个病毒分离株的全长基因组进行了测序，对14个全基因组的系统发育分析重现了Rep基因的分析结果。GRBaV基因组的比较揭示了进化枝1中GRBaV基因组之间一些变异背后的重组证据。对单链DNA病毒的外壳蛋白和复制酶相关蛋白序列进行系统发育分析，结果显示GRBaV属于双生病毒科。这种分类与纳米病毒科和圆环病毒科的成员不同，与已确认的属和新型植物感染性双生病毒样病毒的亲缘关系有限。
