利用单克隆抗体对HLA表位进行血清学定位及其通过测序的HLA分子进行解读。
Serological mapping of HLA-epitopes with monoclonal antibodies and its interpretation by sequenced HLA-molecules.
作者信息
Harpprecht J, Olde K, Westphal E, Müller-Ruchholtz W
机构信息
Department of Immunology, University of Kiel, FRG.
出版信息
Tissue Antigens. 1989 Sep;34(3):170-8. doi: 10.1111/j.1399-0039.1989.tb01733.x.
For the study of the epitope distribution on HLA class I molecules, blocking studies were carried out with a competitive radioimmuno assay using 3H labelled monoclonal antibodies (MAbs) against various HLA epitopes. The results were discussed with the help of known HLA amino acid sequences, and the positions of the following epitopes were suggested: A2/Aw69; A2/28; A2/11/25/26/28/29/30/31/Aw33/34; A25/32; Bw4; B7/Bw22,42; Bw6.
为了研究HLA I类分子上的表位分布，采用针对各种HLA表位的3H标记单克隆抗体（MAbs），通过竞争性放射免疫测定法进行了阻断研究。借助已知的HLA氨基酸序列对结果进行了讨论，并推测了以下表位的位置：A2/Aw69；A2/28；A2/11/25/26/28/29/30/31/Aw33/34；A25/32；Bw4；B7/Bw22,42；Bw6。
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