Nishikawa K, Fukuhara N, Liprandi F, Green K, Kapikian A Z, Chanock R M, Gorziglia M
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Virology. 1989 Dec;173(2):631-7. doi: 10.1016/0042-6822(89)90575-8.
The complete VP4 gene of porcine rotavirus strain OSU has been inserted into a baculovirus expression vector under the control of the polyhedrin promoter. The VP4 outer capsid protein, which is a major neutralization antigen in rotavirus, was expressed in high yield in Spodoptera frugiperda cells. Reactivity with polyclonal and monoclonal antibodies suggested that neutralizing epitopes were functionally unaltered on the expressed VP4. The VP4 produced in this system also induced antibodies in guinea pigs which inhibited hemagglutination of OSU and neutralized its infectivity to high titer. The available evidence suggests that the VP4 expressed in insect cells maintained its antigenic configuration and may prove useful in elucidation of (1) the extent of VP4 polymorphism among human and animal rotaviruses and (2) the distribution of VP4 among these viruses.
猪轮状病毒OSU株的完整VP4基因已被插入到受多角体蛋白启动子控制的杆状病毒表达载体中。VP4外衣壳蛋白是轮状病毒中的主要中和抗原，在草地贪夜蛾细胞中高产量表达。与多克隆和单克隆抗体的反应性表明，表达的VP4上的中和表位在功能上未改变。该系统产生的VP4还在豚鼠中诱导产生抗体，这些抗体抑制OSU的血凝反应并高效中和其感染性。现有证据表明，在昆虫细胞中表达的VP4保持了其抗原构型，可能有助于阐明：（1）人和动物轮状病毒中VP4多态性的程度；（2）VP4在这些病毒中的分布情况。
