Varga G, Solomon T E, Papp M
Institute of Experimental Medicine, Hungarian Academy of Science, Budapest.
Acta Physiol Hung. 1989;73(2-3):319-21.
The purpose of this study was to estimate the effects of cholecystokinin (CCK), somatostatin (SS) pancreatic polypeptide (PP) and their interaction with each other, given them in single doses, on pancreatic secretion and pancreatic growth after long-term treatment in rats. The acute secretory effects of the above mentioned peptides were studied on conscious rats supplied with pancreatic, gastric and jugular vein cannulae. The pancreatic growth was characterized by measurements of pancreatic weight, desoxyribonucleic acid (DNA), protein, trypsin and amylase content after 5 days treatment. Amylase output was increased by caerulein alone, and given it in combination with somatostatin (SS), while its value decreased by SS alone. After 5 days treatment, the pancreatic weight, trypsin and amylase activity (hypertrophy) was increased by caerulein, and these values were not altered by S alone. In combinative administration of caerulein with somatostatin, the stimulatory effect by caerulein was decreased. PP given alone or in combination with caerulein decreased both the basal and stimulated amylase output. PP given for 5 days decreased pancreatic trypsin and amylase contents and counteracted the stimulatory effect by caerulein to these enzymes' contents. It has been concluded that: 1. caerulein stimulates both pancreatic enzyme secretion and pancreatic growth; 2. somatostatin inhibits the pancreatic secretion and caerulein induced pancreatic growth, but it does not affect the spontaneous growth of pancreas; 3. pancreatic polypeptide inhibits the pancreatic secretion and decreases pancreatic trypsin and amylase contents.
本研究的目的是评估在大鼠长期治疗后，单次给予胆囊收缩素（CCK）、生长抑素（SS）、胰多肽（PP）及其相互作用对胰腺分泌和胰腺生长的影响。在有意识的、植入了胰腺、胃和颈静脉插管的大鼠身上研究了上述肽的急性分泌作用。通过在治疗5天后测量胰腺重量、脱氧核糖核酸（DNA）、蛋白质、胰蛋白酶和淀粉酶含量来表征胰腺生长。单独使用蛙皮素可增加淀粉酶分泌量，与生长抑素（SS）联合使用时也可增加，而单独使用SS时其值降低。治疗5天后，蛙皮素可增加胰腺重量、胰蛋白酶和淀粉酶活性（肥大），单独使用S时这些值未改变。蛙皮素与生长抑素联合给药时，蛙皮素的刺激作用减弱。单独给予PP或与蛙皮素联合给予时，均可降低基础和刺激后的淀粉酶分泌量。给予PP 5天可降低胰腺胰蛋白酶和淀粉酶含量，并抵消蛙皮素对这些酶含量的刺激作用。得出以下结论：1. 蛙皮素可刺激胰腺酶分泌和胰腺生长；2. 生长抑素抑制胰腺分泌和蛙皮素诱导的胰腺生长，但不影响胰腺的自发生长；3. 胰多肽抑制胰腺分泌并降低胰腺胰蛋白酶和淀粉酶含量。
