Lucier G E, Egizii R
University of Calgary, Faculty of Medicine, Dept. of Physiology, Alberta, Canada.
J Chem Neuroanat. 1988 Nov-Dec;1(6):303-10.
The afferents of the nasal receptors responsible for many upper airway protective reflexes are carried in the ethmoidal branch of the ophthalmic division of the trigeminal nerve. Previous electrophysiological studies indicate that a significant number of ethmoidal afferents respond to noxious stimuli applied to the nose. The objective of the present study was to identify ethmoidal nerve cell bodies within the trigeminal ganglion which demonstrated the presence of the neurotransmitter substance P (SP). SP is believed to be involved in the relay of nociceptive information. A double-labelling technique was employed and involved tracing the afferents to their cell bodies using horseradish peroxidase (HRP) and subsequent identification of SP-immunoreactivity within HRP-filled cells using monoclonal antibody immunohistochemistry. SP-immunoreactive cell bodies constituted 43 per cent-50 per cent of the total number of labelled ethmoidal cell bodies within the trigeminal ganglion. Although ethmoidal cell bodies were much smaller than the overall population of trigeminal ganglion cells, the size of SP-immunoreactive ethmoidal cell bodies was not significantly different from that of ethmoidal cell bodies not exhibiting SP-immunoreactivity.
负责许多上呼吸道保护性反射的鼻感受器传入神经由三叉神经眼支的筛骨分支传导。先前的电生理研究表明，大量筛骨传入神经对施加于鼻子的有害刺激有反应。本研究的目的是在三叉神经节内识别出显示神经递质P物质（SP）存在的筛骨神经细胞体。SP被认为参与伤害性信息的传递。采用了双标记技术，包括使用辣根过氧化物酶（HRP）将传入神经追溯到其细胞体，随后使用单克隆抗体免疫组织化学在HRP填充的细胞内鉴定SP免疫反应性。SP免疫反应性细胞体占三叉神经节内标记的筛骨细胞体总数的43%-50%。虽然筛骨细胞体比三叉神经节细胞的总体小得多，但显示SP免疫反应性的筛骨细胞体的大小与未显示SP免疫反应性的筛骨细胞体的大小没有显著差异。
