Moungthong Greetha, Klamkam Pana, Mahakit Prasit, Chalermwatanachai Thanit, Thunyaharn Sudaluck, Monyakul Veerapol
Department of Otolaryngology, Phramongkutklao Hospital, Bangkok 10400, Thailand.
Department of Microbiology, Phramongkutklao College of Medicine, Bangkok 10400, Thailand.
Asia Pac Allergy. 2014 Apr;4(2):113-8. doi: 10.5415/apallergy.2014.4.2.113. Epub 2014 Apr 29.
Nowadays, there are many methods to reduce microorganisms in the air, such as dehumidifier, air purifier or humidity and temperature controller. The Precise Climate Controller is an instrument for controlling humidity and temperature, a concept that is demonstrated.
To determine the efficacy of this device, in order to reduce the quantity of the fungi and bacteria in the closed system.
This study is a perspective experimental study and is conducted as follows - the air sample in the closed system, a 42-cubic-meter room, is collected before the installation of the Precise Climate Controller. Next, the room is fumed with Aspergillus flavus and closed for 2 days. Then the instrument is in use in order to keep the relative humidity (RH) and the temperature constant at 55% RH and 25 degrees Celsius (℃). The air samples are collected every 3 days for 5 times during the period of 15 days to identify the type and calculate the quantity of the microorganisms.
Before the Precise Climate Controller has been installed. Three species of bacteria are found in the air samples, but none of the fungus exists in the testing room. Once the room has been fumed with a large amount of A. flavus and the instrument is in use for 3 days, nine colonies of A. flavus are identified, but later on when the instrument is in use for 6, 9, 12, and 15 days, the air samples contain neither fungus nor bacteria.
After keeping the RH and temperature of the closed system constant at 55% RH and 25℃ by using the Precise Climate Controller, it is found that the efficaciousness in controlling the quantity and species of fungi and bacteria is clinically significant.
如今，有许多方法可减少空气中的微生物，如除湿机、空气净化器或湿度和温度控制器。精确气候控制器是一种用于控制湿度和温度的仪器，这一概念已得到验证。
确定该设备的功效，以减少封闭系统中真菌和细菌的数量。
本研究是一项前瞻性实验研究，具体如下进行——在安装精确气候控制器之前，收集封闭系统（一个42立方米的房间）中的空气样本。接下来，用黄曲霉对房间进行熏蒸并封闭2天。然后使用该仪器将相对湿度（RH）和温度保持在55%RH和25摄氏度（℃）恒定。在15天内每3天收集一次空气样本，共收集5次，以鉴定微生物的类型并计算其数量。
在安装精确气候控制器之前，空气样本中发现了三种细菌，但测试房间中未发现真菌。一旦房间用大量黄曲霉熏蒸且仪器使用3天后，鉴定出9个黄曲霉菌落，但后来在仪器使用6、9、12和15天时，空气样本中既没有真菌也没有细菌。
通过使用精确气候控制器将封闭系统的RH和温度保持在55%RH和25℃恒定后，发现其在控制真菌和细菌数量及种类方面的有效性具有临床意义。
