Bondarenko O I
Fiziol Zh (1994). 2014;60(1):64-9.
Mitochondria play a central role in the regulation of cell function. Ca2+ entry into mitochondria represents a central event in the regulation of ATP production, intracellular Ca2+ homesotasis and mitochondrial permeability transition pore opening. Until recently, our understanding of the mechanisms of mitochondria Ca2+ uptake was largely limited by the use of indirect optical methods. Utilization of patch-clamp method to isolated mitoplasts allowed characterizing single channel properties of Ca2+ permeable channels in the inner mitochondrial membrane. Here we show the presence of three types of single channel activities of Ca2+ permeable channels in mitoplasts isolated from endothelial cells. While the identity and molecular structure of these channels still remain to be identified, these findings point for complex mechanisms of Ca2+ entry in mitochondria.
线粒体在细胞功能调节中起着核心作用。钙离子进入线粒体是ATP生成调节、细胞内钙离子稳态及线粒体通透性转换孔开放过程中的核心事件。直到最近，我们对线粒体钙离子摄取机制的理解在很大程度上受限于间接光学方法的使用。利用膜片钳技术对分离的线粒体进行研究，能够表征线粒体内膜上钙离子通透通道的单通道特性。在此，我们展示了从内皮细胞分离的线粒体中存在三种类型的钙离子通透通道单通道活性。虽然这些通道的特性和分子结构仍有待确定，但这些发现表明线粒体中钙离子进入的机制很复杂。
