Wang Zheng-Rong, Bao Huai-En
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Apr;31(2):104-7.
To study the effect of Toxoplasma gondii prugniaud strain infection on female reproductive toxicity in mice and learning ability of their F1 generation.
Thirteen ICR mice were each infected intragastrically with 10 T. gondii cysts on the 15th day of pregnancy (late stage pregnancy). 12 mice were treated with physiological saline as control. The time from conception to birth and the number of offspring were recorded. Three mice from each group were sacrificed when pregnant 20 d, placentas from the sacrificed and output stillbirth mice were examined by using histopathology and immunohistochemistry. DNA extraction was performed from placenta tissue, and then T. gondii B1 gene was amplified by PCR. The F1 generation mice from experiment group and control group were tested by Morris water maze test. Statistical analysis on learning and memory ability was made by SPSS 13.0 software.
The time from conception to birth in experiment group [(19.2 +/- 1.751)d] was shorter than that in control group [(21.0 +/- 1.732)d] (P < 0.05). No significant difference was found in the number of offspring between experiment group (70) and control group (85) (P > 0.05). Microscopic examination with HE staining showed multiple T. gondii among placental villi, the increase of the number of Hofbauer cells, blood sinus expansion and hyperemia, and visible nucleated erythrocytes. Immunohistochemically, T. gondii antigen was detected in placenta tissue. T. gondii B1 gene was detected in placenta tissue (194 bp). On the third and fourth day of the Morris water maze test, the latency of experiment group [(29.92 +/- 4.28) s, (27.69 +/- 6.23) s] was longer than that of the control [(24.07 +/- 5.32) s, (22.25 +/- 7.94) s] (P < 0.05). In the spatial probe test, the distance across the platform quadrant of experiment group [(384.66 +/- 41.33) cm] was shorter than that of the control [(426.12 +/- 46.48) cm] (P < 0.05).
T. gondii Prugniaud strain infection in late stage pregnancy of mice may induce reproductive toxicity and affect the learning and memory capability of the F1 generation.
研究刚地弓形虫普氏株感染对小鼠雌性生殖毒性及其F1代学习能力的影响。
13只ICR小鼠在妊娠第15天（妊娠晚期）经口灌胃感染10个刚地弓形虫包囊。12只小鼠用生理盐水处理作为对照。记录受孕至分娩时间及仔鼠数量。妊娠20 d时每组处死3只小鼠，对处死小鼠及产出的死胎小鼠的胎盘进行组织病理学和免疫组织化学检查。从胎盘组织中提取DNA，然后通过PCR扩增刚地弓形虫B1基因。对实验组和对照组的F1代小鼠进行Morris水迷宫试验。用SPSS 13.0软件对学习和记忆能力进行统计学分析。
实验组受孕至分娩时间[（19.2±1.751）d]短于对照组[（21.0±1.732）d]（P<0.05）。实验组（70只）与对照组（85只）仔鼠数量差异无统计学意义（P>0.05）。HE染色显微镜检查显示胎盘绒毛中有多个刚地弓形虫，霍夫鲍尔细胞数量增加，血窦扩张、充血，可见有核红细胞。免疫组织化学检测胎盘组织中有刚地弓形虫抗原。在胎盘组织中检测到刚地弓形虫B1基因（194 bp）。在Morris水迷宫试验的第3天和第4天，实验组潜伏期[（29.92±4.28）s，（27.69±6.23）s]长于对照组[（24.07±5.32）s，（22.25±7.94）s]（P<0.05）。在空间探索试验中，实验组穿过平台象限的距离[（384.66±41.33）cm]短于对照组[（426.12±46.48）cm]（P<0.05）。
小鼠妊娠晚期感染刚地弓形虫普氏株可能诱发生殖毒性并影响F1代的学习和记忆能力。
