Zhou Rui-Min, Zhang Hong-Wei, Deng Yan, Qian Dan, Liu Ying, Chen Wei-Qi, Yan Qiu-Ye, Su Yun-Pu, Zhao Xu-Dong, Xu Bian-Li
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Apr;31(2):127-30.
To compare the laboratory tests of the imported Plasmodium ovale infection and analyse the genetic character.
After Giemsa staining and microscopy, CareStart rapid detection and nested PCR were used to detect two cases with P. ovale infection returning from Congo (Brazzaville) in Henan Province. Sequencing was performed after PCR amplification using the 18S rRNA genus-specific primers. Their genetic characteristics were analyzed and the sequence homology analysis was performed in the NCBI.
The two cases were confirmed as P. ovale infection by morphological examination microscopically. Amplified bands were produced by 18S rRNA nested PCR, which was the same with P. ovale in size, whereas the results of CareStart rapid detection test were all negative. A sequence of 906 bp in length was obtained by sequencing their 18S rRNA genes in which GC accounted for 35.4%, and the sequence showed 99% homology to the corresponding part of the known P. ovale 18S rRNA gene (GenBank accession No. AB182492).
Both the nested PCR and microscopy confirm the infection of P. ovale. A negative result of CareStart rapid detection can not ruled out the Plasmodium infection.
比较输入性卵形疟原虫感染的实验室检测方法并分析其基因特征。
对河南省2例从刚果（布）回国的卵形疟原虫感染病例进行吉姆萨染色镜检后，采用CareStart快速检测法和巢式PCR进行检测。使用18S rRNA属特异性引物进行PCR扩增后测序。分析其基因特征并在NCBI中进行序列同源性分析。
2例经镜检形态学检查确诊为卵形疟原虫感染。18S rRNA巢式PCR扩增出条带，大小与卵形疟原虫相同，而CareStart快速检测试验结果均为阴性。对其18S rRNA基因测序获得长度为906 bp的序列，其中GC占35.4%，该序列与已知卵形疟原虫18S rRNA基因（GenBank登录号：AB182492）相应部分的同源性为99%。
巢式PCR和镜检均证实卵形疟原虫感染。CareStart快速检测结果为阴性不能排除疟原虫感染。
