Jiang Xuhong, Wang Yuan, Hua Junyi, Lyu Bin
Department of Emergency, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang, China. Corresponding author: Lyu Bin, Email:
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2014 May;26(5):343-6. doi: 10.3760/cma.j.issn.2095-4352.2014.05.012.
To study the antibacterial property of silver loaded titanium dioxide (TiO2) antibacterial coated endotracheal intubation tube, and to determine the minimum effective antibacterial concentration.
Intubation tubes coated with different concentrations of antibacterial agents were prepared with sol gel method. Polyethylene endotracheal intubation tubes were used as substrate, and silver loaded TiO2 was used as the antibacterial agent. According to the different antibacterial concentrations of the antibacterial agent, the tubes were divided into nine groups: 10.0% group, 5.0% group, 2.0% group, 1.5% group, 1.0% group, 0.8% group, 0.6% group, 0.2% group, and control group. They were respectively immersed in three standard bacteria suspensions with 1.0×10(5) cfu/mL: Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Together with standard bacteria liquid group, there were 10 experimental groups. They were kept overnight for 24 hours. 10 μL of respective culture medium was smeared on blood agar culture medium. After being cultured overnight in 35 centigrade, the number of bacteria colonies was respectively counted.
In 1.0×10(5) cfu/mL of three standard bacteria liquids with antibacterial agent concentration ≥1.0%, three bacterial colonies had un-obviously growth rate. Almost the same strong antibacterial effects to achieve sterilizing rates of more than 98% was shown in each group of the antibacterial coating endotracheal intubation tubes (all P>0.05). As the antibacterial agent concentration decreased, three bacterial colonies were increasing gradually. Intermediate antibacterial effects were shown in tubes of 0.8% group, with significant statistic difference as compared with 1.0% and 0.6% groups [Pseudomonas aeruginosa: 7.300 (4.050, 8.350) vs. 0.200 (0.050, 1.200), 9.700(9.000, 10.000); Staphylococcus aureus: 4.100 (3.300, 4.650) vs. 0.000 (0.000, 0.150), 5.800 (5.350, 7.650); Escherichia coli: 1.400 (0.750, 3.750) vs. 0.050 (0.025, 0.050), 9.500 (8.500, 9.800), all P<0.01].
Silver loaded TiO2 antibacterial coated endotracheal intubation tube had definite antibacterial properties, which were related to the antibacterial concentration. Strong antibacterial effects were shown when antibacterial concentration was above 1.0%, with bacteria almost completely killed in the immersing liquid.
研究载银二氧化钛（TiO₂）抗菌涂层气管插管的抗菌性能，并确定其最低有效抗菌浓度。
采用溶胶 - 凝胶法制备涂覆不同浓度抗菌剂的插管。以聚乙烯气管插管为基底，载银TiO₂为抗菌剂。根据抗菌剂不同抗菌浓度，将插管分为九组：10.0%组、5.0%组、2.0%组、1.5%组、1.0%组、0.8%组、0.6%组、0.2%组和对照组。将它们分别浸入三种浓度为1.0×10⁵ cfu/mL的标准菌悬液中：铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌。连同标准菌液组，共10个实验组。放置过夜24小时。各取10 μL相应培养基涂抹于血琼脂培养基上。在35摄氏度培养过夜后，分别计数菌落数。
在抗菌剂浓度≥1.0%的1.0×10⁵ cfu/mL的三种标准菌液中，三种菌落生长率不明显。每组抗菌涂层气管插管均显示出几乎相同的强抗菌效果，杀菌率均超过98%（均P>0.05）。随着抗菌剂浓度降低，三种菌落逐渐增多。0.8%组插管显示出中等抗菌效果，与1.0%组和0.6%组相比有显著统计学差异[铜绿假单胞菌：7.300（4.050，8.350）对0.200（0.050，1.200），9.700（9.000，10.000）；金黄色葡萄球菌：4.100（3.300，4.650）对0.000（0.000，0.150），5.800（5.350，7.650）；大肠杆菌：1.400（0.750，3.750）对0.050（0.025，0.050），9.500（8.500，9.800），均P<0.01]。
载银TiO₂抗菌涂层气管插管具有一定的抗菌性能，且与抗菌浓度有关。抗菌浓度在1.0%以上时显示出强抗菌效果，浸泡液中细菌几乎被完全杀灭。
