Yan Ji-Geng, Logiudice John, Davis John, Zhang Lin-Ling, Agresti Michael, Sanger James, Matloub Hani S, Havlik Robert
Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
Muscle Nerve. 2015 Feb;51(2):229-34. doi: 10.1002/mus.24281. Epub 2014 Nov 19.
After nerve injury, excessive calcium impedes nerve regeneration. We previously showed that calcitonin improved nerve regeneration in crush injury. We aimed to validate the direct effect of calcitonin on transected and repaired nerve.
Two rat groups (n = 8) underwent sciatic nerve transection followed by direct repair. In the calcitonin group, a calcitonin-filled mini-osmotic pump was implanted subcutaneously, with a catheter parallel to the repaired nerve. The control group underwent repair only, without a pump. Evaluation and comparison between the groups included: (1) compound muscle action potential recording of the extensor digitorum longus (EDL) muscle; (2) tetanic muscle force test of EDL; (3) nerve calcium concentration; and (4) nerve fiber count and calcified spot count.
The calcitonin pump group showed superior recovery.
Calcitonin affects injured and repaired peripheral nerve directly. The calcitonin-filled mini-osmotic pump improved nerve functional recovery by accelerating calcium absorption from the repaired nerve. This finding has potential clinical applications.
神经损伤后，过量的钙会阻碍神经再生。我们之前表明降钙素可改善挤压伤中的神经再生。我们旨在验证降钙素对横断并修复的神经的直接作用。
两组大鼠（每组n = 8）接受坐骨神经横断并直接修复。在降钙素组中，皮下植入一个填充降钙素的微型渗透泵，导管与修复的神经平行。对照组仅进行修复，不植入泵。两组之间的评估和比较包括：（1）趾长伸肌（EDL）的复合肌肉动作电位记录；（2）EDL的强直肌力测试；（3）神经钙浓度；以及（4）神经纤维计数和钙化斑计数。
降钙素泵组显示出更好的恢复效果。
降钙素直接影响受损并修复的周围神经。填充降钙素的微型渗透泵通过加速从修复神经中吸收钙来改善神经功能恢复。这一发现具有潜在的临床应用价值。
