Zheleznikova G F, Blagoslovenskiĭ G S, Gnilevskaia Z U
Lab Delo. 1989(10):56-60.
The authors suggest a simple method for studying the lymphocyte blastogenesis (LBG) regulation in whole blood culture, consisting in preincubation of a part of the material and comparison of the results in two variants of the experiment: upon immediate mitogen stimulation and stimulated in 24 hrs after pre-cultivation. LBG activation or suppression is established from the value of the preincubation index.
作者们提出了一种在全血培养中研究淋巴细胞增殖反应(LBG)调节的简单方法,该方法包括对一部分材料进行预孵育,并在实验的两种变体中比较结果:即立即进行有丝分裂原刺激以及在预培养24小时后进行刺激。根据预孵育指数的值来确定LBG的激活或抑制情况。