Lin Long-Ze, Mukhopadhyay Sudarsan, Robbins Rebecca J, Harnly James M
Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, ARS-USDA, Beltsville, MD 20705, USA.
J Food Compost Anal. 2007 Aug 1;20(5):361-369. doi: 10.1016/j.jfca.2006.09.005.
LC-DAD-ESI/MS was used to identify 23 flavonoids in the extract of Mexican oregano ( H.B.K.), a spice and herb, used in the USA and Mexico. The identification of luteolin-7-O-glucoside, apigenin 7-O-glucoside, phloridzin, taxifolin, eriodictyol, scutellarein, luteolin, quercetin, naringenin, pinocembrin and galangin was confirmed by direct comparison with standards. Identification of 6-hydroxyluteolin, two 6-hydroxyluteolin 7-O-glycosides, three pentahydroxyflavanone hexosides, scutellarein 7-O-hexoside, 3-hydroxyphloretin hexoside, and three other flavones, was made by detailed analysis of their UV and mass spectral data. The identification of the flavonoid glycosides was further confirmed through detection of their aglycones following hydrolysis of the samples. The concentration of the identified flavonoids in three samples was also estimated. This is the first report of detection of over 20 flavonoids, including chalcones, in this plant material.
液相色谱 - 二极管阵列检测 - 电喷雾电离质谱法（LC - DAD - ESI/MS）用于鉴定墨西哥牛至（H.B.K.）提取物中的23种黄酮类化合物，墨西哥牛至是一种在美国和墨西哥使用的香料和草药。通过与标准品直接比较，确认了木犀草素 - 7 - O - 葡萄糖苷、芹菜素7 - O - 葡萄糖苷、根皮苷、紫杉叶素、圣草酚、黄芩素、木犀草素、槲皮素、柚皮素、松属素和高良姜素的鉴定。通过对其紫外和质谱数据的详细分析，鉴定出了6 - 羟基木犀草素、两种6 - 羟基木犀草素7 - O - 糖苷、三种五羟基黄酮己糖苷、黄芩素7 - O - 己糖苷、3 - 羟基根皮素己糖苷以及其他三种黄酮。通过对样品水解后苷元的检测，进一步证实了黄酮苷的鉴定。还估算了三个样品中已鉴定黄酮类化合物的浓度。这是首次报道在这种植物材料中检测到20多种黄酮类化合物，包括查耳酮。
