经仿生处理的生物活性复合材料增强的成骨能力

Enhanced osteogenicity of bioactive composites with biomimetic treatment.

作者信息

Meretoja Ville V, Tirri Teemu, Malin Minna, Seppälä Jukka V, Närhi Timo O

机构信息

Department of Prosthetic Dentistry, University of Turku, Lemminkäisenkatu 2, 20520 Turku, Finland ; Turku Clinical Biomaterials Centre, University of Turku, Itäinen Pitkäkatu 4B, 20520 Turku, Finland.

Polymer Technology, School of Chemical Technology, Aalto University, P.O. Box 16100, Aalto, 00076 Espoo, Finland.

出版信息

Biomed Res Int. 2014;2014:207676. doi: 10.1155/2014/207676. Epub 2014 Apr 9.

DOI:10.1155/2014/207676

PMID:24812608

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4000935/

Abstract

PURPOSE

This study aimed to explore if initiation of biomimetic apatite nucleation can be used to enhance osteoblast response to biodegradable tissue regeneration composite membranes.

MATERIALS AND METHODS

Bioactive thermoplastic composites consisting of poly(ε-caprolactone/DL-lactide) and bioactive glass (BAG) were prepared at different stages of biomimetic calcium phosphate deposition by immersion in simulated body fluid (SBF). The modulation of the BAG dissolution and the osteogenic response of rat mesenchymal stem cells (MSCs) were analyzed.

RESULTS

SBF treatment resulted in a gradual calcium phosphate deposition on the composites and decreased BAG reactivity in the subsequent cell cultures. Untreated composites and composites covered by thick calcium phosphate layer (14 days in SBF) expedited MSC mineralization in comparison to neat polymers without BAG, whereas other osteogenic markers--alkaline phosphatase activity, bone sialoprotein, and osteocalcin expression--were initially decreased. In contrast, surfaces with only small calcium phosphate aggregates (five days in SBF) had similar early response than neat polymers but still demonstrated enhanced mineralization.

CONCLUSION

A short biomimetic treatment enhances osteoblast response to bioactive composite membranes.

摘要

目的

本研究旨在探讨仿生磷灰石成核的启动是否可用于增强成骨细胞对可生物降解组织再生复合膜的反应。

材料与方法

通过浸泡在模拟体液（SBF）中，在仿生磷酸钙沉积的不同阶段制备由聚（ε-己内酯/DL-丙交酯）和生物活性玻璃（BAG）组成的生物活性热塑性复合材料。分析了BAG溶解的调节和大鼠间充质干细胞（MSCs）的成骨反应。

结果

SBF处理导致复合材料上逐渐沉积磷酸钙，并降低了后续细胞培养中BAG的反应性。与不含BAG的纯聚合物相比，未处理的复合材料和被厚磷酸钙层覆盖的复合材料（在SBF中处理14天）加速了MSC矿化，而其他成骨标志物——碱性磷酸酶活性、骨唾液蛋白和骨钙素表达——最初有所下降。相比之下，只有少量磷酸钙聚集体的表面（在SBF中处理5天）与纯聚合物具有相似的早期反应，但仍显示出矿化增强。