ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Biomaterials for Health Research Group, Institut des matériaux I-MAT (FD4122), Université de Cergy-Pontoise, Maison Internationale de la Recherche (MIR), rue Descartes, 95001, Neuville sur Oise Cedex, France.
Laboratory of Biomaterials and Tissue Engineering, Faculty of Medicine and Health Technology, Tampere University, Korkeakoulunkatu 3, 33720, Tampere, Finland.
Mater Sci Eng C Mater Biol Appl. 2020 Feb;107:110340. doi: 10.1016/j.msec.2019.110340. Epub 2019 Oct 24.
Bioactive glass (BAG)/Poly (Lactic Acid) (PLA) composites have great potential for bone tissue engineering. The interest in these materials is to obtain a scaffold with tailorable properties bringing together the advantages of the composites' constituents such as the biodegradability, bioactivity and osteoinduction. The materials studied are PLA/13-93 and PLA/13-93B20 (20% of SiO is replaced with BO in the 13-93 composition). To characterize them, they were dissolved in TRIS buffer and Simulated Body Fluid (SBF) in vitro. Over the 10 weeks of immersion in TRIS, the ion release from the composites was constant. Following immersion in SBF for 2 weeks, the hydroxyapatite (HA) layer was found to precipitate at the composites surface. By adding Boron, both these reactions were accelerated, as the borosilicate glass dissolves faster than pure silicate glass alone. Polymer degradation was studied and showed that during immersion, the pure PLA rods maintained their molecular weight whereby the composites decreased with time, but despite this the mechanical properties remained stable for at least 10 weeks. Their ability to induce osteogenic differentiation of myoblastic cells was also demonstrated with cell experiments showing that C2C12 cells were able to proliferate and spread on the composites. The Myosin Heavy Chain and Osteopontin were tracked by immunostaining the cells and showed a suppression of the myosin signal and the presence of osteopontin, when seeded onto the composites. This proves osteoinduction occurred. In studying the mineralization of the cells, it was found that BAG presence conditions the synthesizing of mineral matter in the cells. The results show that these composites have a potential for bone tissue engineering.
生物活性玻璃（BAG）/聚乳酸（PLA）复合材料在骨组织工程中有很大的应用潜力。人们对这些材料感兴趣是因为希望获得一种支架，这种支架具有可调节的特性，结合了复合材料成分的优点，如可生物降解性、生物活性和骨诱导性。研究的材料为 PLA/13-93 和 PLA/13-93B20（13-93 组成中 20%的 SiO 被 BO 取代）。为了对它们进行表征，将它们溶解在三羟甲基氨基甲烷缓冲液和模拟体液（SBF）中进行体外实验。在 TRIS 中浸泡的 10 周内，复合材料的离子释放量保持不变。在 SBF 中浸泡 2 周后，发现复合材料表面沉淀出羟基磷灰石（HA）层。通过添加硼，这两种反应都得到了加速，因为硼硅酸盐玻璃的溶解速度比纯硅酸盐玻璃快。研究了聚合物的降解情况，结果表明在浸泡过程中，纯 PLA 棒保持其分子量，而复合材料随时间减少，但尽管如此，其机械性能至少在 10 周内保持稳定。通过细胞实验也证明了它们诱导成肌细胞成骨分化的能力，实验表明 C2C12 细胞能够在复合材料上增殖和扩散。通过免疫染色追踪肌球蛋白重链和骨桥蛋白，结果表明当细胞接种到复合材料上时，肌球蛋白信号受到抑制，而骨桥蛋白存在。这证明了骨诱导的发生。在研究细胞矿化时，发现 BAG 的存在条件决定了细胞中矿物质的合成。结果表明，这些复合材料在骨组织工程中有应用潜力。
