Servicio de Microbiología y Tuberculosis, Hospital Vozandes, Quito, Ecuador; Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador; Unidad de Investigaciones en Biomedicina, Zurita & Zurita Laboratorios, Quito, Ecuador.
Clin Microbiol Infect. 2014 Dec;20(12):O1113-6. doi: 10.1111/1469-0691.12675. Epub 2014 Jul 16.
Mycobacterium timonense is a non-tuberculous mycobacteria (NTM) described in southern France in 2009, and to our knowledge, not reported again as a human pathogen in indexed literature. The aim of this work was to characterize the first clinical isolate of M. timonense in Ecuador. Time of growth, biochemical tests, thin layer growth test, PCR-RFLP analysis of the hsp65 gene and MALDI-TOF spectra analysis were not able to identify the species. The species identification was achieved through sequencing of rrs, hsp65 and rpoB genes. The results highlight the necessity to set up a sequencing method to identify emerging NTM in Ecuadorian clinical facilities.
蒂姆诺分枝杆菌是 2009 年在法国南部发现的一种非结核分枝杆菌（NTM），据我们所知，在索引文献中尚未再次报道其为人类病原体。本研究的目的是对厄瓜多尔首例临床分离的蒂姆诺分枝杆菌进行鉴定。生长时间、生化试验、薄层生长试验、hsp65 基因 PCR-RFLP 分析和 MALDI-TOF 图谱分析均无法鉴定该菌种。通过 rrs、hsp65 和 rpoB 基因测序实现了菌种鉴定。研究结果强调了在厄瓜多尔临床机构建立测序方法以鉴定新型 NTM 的必要性。
