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一个短的RNA茎环对于锥虫对数早期阶段的基因表达抑制是必要且充分的。

A short RNA stem-loop is necessary and sufficient for repression of gene expression during early logarithmic phase in trypanosomes.

作者信息

Fernández-Moya Sandra M, Carrington Mark, Estévez Antonio M

机构信息

Instituto de Parasitología y Biomedicina 'López-Neyra', IPBLN-CSIC, Parque Tecnológico de Ciencias de la Salud, Avda. del Conocimiento, s/n, 18016 Armilla, Granada, Spain.

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.

出版信息

Nucleic Acids Res. 2014 Jun;42(11):7201-9. doi: 10.1093/nar/gku358. Epub 2014 May 9.

DOI:10.1093/nar/gku358
PMID:24813448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4066783/
Abstract

We have compared the transcriptomes of cultured procyclic Trypanosoma brucei cells in early and late logarithmic phases and found that ∼200 mRNAs were differentially regulated. In late log phase cells, the most upregulated mRNA encoded the nucleobase transporter NT8. The 3' untranslated region (UTR) of NT8 contains a short stem-loop cis-element that is necessary for the regulation of NT8 expression in response to external purine levels. When placed in the 3'-UTR of an unregulated transcript, the cis-element is sufficient to confer regulation in response to purines. To our knowledge, this is the first example of a discrete RNA element that can autonomously regulate gene expression in trypanosomes in response to an external factor and reveals an unprecedented purine-dependent signaling pathway that controls gene expression in eukaryotes.

摘要

我们比较了处于对数生长期早期和晚期的培养前循环型布氏锥虫细胞的转录组，发现约200种mRNA受到差异调节。在对数生长期后期的细胞中，上调最明显的mRNA编码核碱基转运蛋白NT8。NT8的3'非翻译区（UTR）包含一个短茎环顺式元件，该元件对于响应外部嘌呤水平调节NT8表达是必需的。当置于未受调节的转录本的3'-UTR中时，该顺式元件足以赋予对嘌呤的调节作用。据我们所知，这是一个离散RNA元件的首个例子，该元件可自主调节锥虫中因外部因素引起的基因表达，并揭示了一条前所未有的控制真核生物基因表达的嘌呤依赖性信号通路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/1c821cf01b67/gku358fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/fae037a60124/gku358fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/0f4d26bcf61d/gku358fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/bf86f7e9efcb/gku358fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/b273b8c18f2e/gku358fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/73b667ccd6e9/gku358fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/7860b919eca2/gku358fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/1c821cf01b67/gku358fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/fae037a60124/gku358fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/0f4d26bcf61d/gku358fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/bf86f7e9efcb/gku358fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/b273b8c18f2e/gku358fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/73b667ccd6e9/gku358fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/7860b919eca2/gku358fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090a/4066783/1c821cf01b67/gku358fig7.jpg

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