Toll样受体2的Arg677Trp和Arg753Gln多态性、Toll样受体4的Asp299Gly多态性与巨细胞病毒感染之间的关系。
Relationship between toll-like receptor 2 Arg677Trp and Arg753Gln and toll-like receptor 4 Asp299Gly polymorphisms and cytomegalovirus infection.
作者信息
Jabłońska Agnieszka, Paradowska Edyta, Studzińska Mirosława, Suski Patrycja, Nowakowska Dorota, Wiśniewska-Ligier Małgorzata, Woźniakowska-Gęsicka Teresa, Wilczyński Jan, Leśnikowski Zbigniew J
机构信息
Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland.
Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland.
出版信息
Int J Infect Dis. 2014 Aug;25:11-5. doi: 10.1016/j.ijid.2014.04.001. Epub 2014 May 9.
OBJECTIVES
The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults.
METHODS
The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. The viral load was measured by quantitative real-time PCR.
RESULTS
Almost all of the patients with CMV infections were wild-type homozygotes without TLR2 and TLR4 polymorphisms. No significant differences in TLR2 and TLR4 polymorphisms were observed between infants with or without CMV infection. Compared with adults with CMV infections, heterozygosity for the TLR2 Arg677Trp and TLR4 Asp299Gly SNPs was detected more frequently in healthy individuals (p<0.05). Logistic regression analysis showed that the wild-type TLR2 genotype was associated with an increased risk of CMV infection and that heterozygosity for TLR2 and TLR4 SNPs diminished the risk of CMV infection in adult patients. An association between CMV load and the TLR4 SNP was found.
CONCLUSION
Our results suggest that the wild-type TLR2 genotype may be a risk factor for CMV replication in adult patients.
目的
在婴儿和成人中研究Toll样受体2（TLR2，Arg677Trp、Arg753Gln）及Toll样受体4（TLR4，Asp299Gln）中特定单核苷酸多态性（SNP）与人类巨细胞病毒（CMV）感染之间的关联。
方法
对151例CMV感染患者及78名无亲缘关系的健康个体进行TLR2和TLR4基因分型。采用聚合酶链反应（PCR）扩增片段的限制性片段长度多态性（RFLP）分析进行基因分型。通过定量实时PCR检测病毒载量。
结果
几乎所有CMV感染患者均为无TLR2和TLR4基因多态性的野生型纯合子。有无CMV感染的婴儿之间，TLR2和TLR4基因多态性无显著差异。与CMV感染的成人相比，健康个体中TLR2 Arg677Trp和TLR4 Asp299Gly SNP杂合子的检出频率更高（p<0.05）。逻辑回归分析显示，野生型TLR2基因型与CMV感染风险增加相关，TLR2和TLR4 SNP杂合子可降低成年患者CMV感染风险。发现CMV载量与TLR4 SNP之间存在关联。
结论
我们的结果表明，野生型TLR2基因型可能是成年患者CMV复制的危险因素。
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