Chao L, Chen Y P, Woodley-Miller C, Li L Y, von Harten K, Chao J
Department of Biochemistry, Medical University of South Carolina, Charleston.
Adv Exp Med Biol. 1989;247A:73-80. doi: 10.1007/978-1-4615-9543-4_11.
A renal kallikrein gene has been isolated, sequenced and characterized from a rat genomic library using a kallikrein cDNA probe. The kallikrein gene is 4160 bases in length and consists of 5 exons and 4 introns. The deduced sequence of the gene encodes an 18 amino acid (a.a.) signal peptide, a 6 a.a. propeptide and a 237 a.a. active enzyme with an N-terminal valine. A sequence comparison of this and tissue kallikrein (pancreatic kallikrein) indicates the key a.a. residues for serine protease activity (HIS-ASP-SER) and cleavage specificity at basic a.a. Northern blot analysis using a specific oligonucleotide probe reveals that this gene is expressed specifically in the kidney but not in the pancreas. This gene is also expressed non-specifically in the submandibular gland which expresses all kallikrein family genes. The expression of this kidney-specific kallikrein gene is regulated by steroid hormones.
利用激肽释放酶cDNA探针，从大鼠基因组文库中分离、测序并鉴定了一个肾激肽释放酶基因。该激肽释放酶基因长度为4160个碱基，由5个外显子和4个内含子组成。该基因推导的序列编码一个18个氨基酸的信号肽、一个6个氨基酸的前肽和一个含N端缬氨酸的237个氨基酸的活性酶。将此基因与组织激肽释放酶（胰激肽释放酶）进行序列比较，结果表明丝氨酸蛋白酶活性的关键氨基酸残基（组氨酸-天冬氨酸-丝氨酸）以及在碱性氨基酸处的切割特异性。使用特异性寡核苷酸探针进行的Northern印迹分析显示，该基因在肾脏中特异性表达，而在胰腺中不表达。该基因在颌下腺中也有非特异性表达，颌下腺表达所有激肽释放酶家族基因。这种肾脏特异性激肽释放酶基因的表达受类固醇激素调控。
