Hattori Mitsuru, Ozawa Takeaki
Department of Chemistry, School of Science, The University of Tokyo.
Anal Sci. 2014;30(5):539-44. doi: 10.2116/analsci.30.539.
Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein-protein interactions and their related intracellular signaling in living cells. This review presents a summary of recent topics for the analysis of biological events using split luciferase complementation techniques. Different applications such as G-protein-coupled receptor (GPCR) screening, monitoring phosphorylations, protein-protein interactions, and pH differences in living tissues are described.
诸如荧光素酶之类的生物发光蛋白是具有高灵敏度和宽动态范围的独特分析工具。然而，这些蛋白的适用性一直局限于报告基因分析。分裂荧光素酶互补技术是一种用于检测活细胞中蛋白质-蛋白质相互作用及其相关细胞内信号传导的强大新策略。本综述总结了使用分裂荧光素酶互补技术分析生物事件的近期研究课题。描述了不同的应用，如G蛋白偶联受体（GPCR）筛选、监测磷酸化、蛋白质-蛋白质相互作用以及活组织中的pH差异。
